สืบค้นงานวิจัย
Single nucleotide polymorphism-based multiplex PCR for identification and genotyping of the oomycete Pythium insidiosum from humans, animals and the environment
Rujirawat T. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Single nucleotide polymorphism-based multiplex PCR for identification and genotyping of the oomycete Pythium insidiosum from humans, animals and the environment
ผู้แต่ง / หัวหน้าโครงการ (EN): Rujirawat T.
บทคัดย่อ (EN): Pythium insidiosum causes a life-threatening infectious disease, called pythiosis, in humans and animals worldwide. Diagnosis of pythiosis is difficult and often delayed. Surgical removal of infected tissue is the main treatment option. Disabilities and death are common outcomes for pythiosis patients. Reports of Py. insidiosum infections are rising. While it would be useful for clinical, epidemiological, and microbiological studies, information on genetic variation in Py. insidiosum strains is limited. This limitation is, at least in part, due to the cost and time-requirements of DNA sequencing procedures. rDNA-sequence-based phylogenetic analyses categorize Py. insidiosum into three groups, in relation to geographic distribution: Clade-I (American strains), Clade-II (American, Asian, and Australian strains), and Clade-III (Thai and American strains). In rDNA sequence analyses, we observed single nucleotide polymorphisms (SNP) that were associated with the phylogenetic clades of Py. insidiosum. In this study, we aim to develop a multiplex PCR assay, targeting the identified SNPs, for rapid genotyping of Py. insidiosum. We also aim to assess diagnostic efficiency of the assay for identification of Py. insidiosum. Fifty-three isolates of Py. insidiosum from humans (n = 35), animals (n = 14), and the environment (n = 4), and 22 negative-control fungi were recruited for assay evaluation. Based on the pattern of amplicons, the multiplex PCR correctly assigned phylogenetic clades in 98% of the Py. insidiosum isolates tested. The assay exhibited 100% sensitivity and specificity for identification of Py. insidiosum. The assay successfully identified and genotyped the first proven isolate of Py. insidiosum from an animal with pythiosis in Thailand. In conclusion, the multiplex PCR provided accurate, sensitive and specific results for identifying and genotyping Py. insidiosum. Thus, this multiplex-PCR assay could be a simple, rapid, and cost-effective alternative to DNA sequencing for the identification and genotyping of Py. insidiosum. © 2017 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028316472&doi=10.1016%2fj.meegid.2017.08.004&partnerID=40&md5=cf1e6aadadee9de8f3dc92013d5ccfe9
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Pythium
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Rujirawat T.. "Single nucleotide polymorphism-based multiplex PCR for identification and genotyping of the oomycete Pythium insidiosum from humans, animals and the environment". ไม่ระบุหน่วยงาน. ม.ป.ป.

Rujirawat T.. "Single nucleotide polymorphism-based multiplex PCR for identification and genotyping of the oomycete Pythium insidiosum from humans, animals and the environment". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Single nucleotide polymorphism-based multiplex PCR for identification and genotyping of the oomycete Pythium insidiosum from humans, animals and the environment
Rujirawat T.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Biochemical and genetic analyses of the oomycete Pythium insidiosum provide new insights into clinical identification and urease-based evolution of metabolismrelated traits Transcriptome analysis reveals pathogenicity and evolutionary history of the pathogenic oomycete Pythium insidiosum Comprehensive Identification of Single Nucleotide Polymorphisms Associated with Beta-lactam Resistance within Pneumococcal Mosaic Genes The immunoreactive exo-1,3-β-glucanase from the pathogenic oomycete pythium insidiosum is temperature regulated and exhibits glycoside hydrolase activity The elicitin-like glycoprotein, ELI025, is secreted by the pathogenic oomycete pythium insidiosum and evades host antibody responses Geographic variation in the elicitin-like glycoprotein, ELI025, of Pythium insidiosum isolated from human and animal subjects Multiplex-PCR As a rapid and sensitive method for identification of meat species in halal-meat products การตรวจหาเชื้อไวรัสและแบคทีเรียก่อโรคอุจจาระร่วงเฉียบพลัน 19 ชนิด ในการทดสอบเดียว ด้วยเทคนิค Multiplex real-time PCR Detection of 19 types of virus and bacteria causing acute diarrhea in single test b Molecular identification of enteroviruses from cattle and goat feces and environment in Thailand การตรวจวิเคราะห์ชนิดไวรัส Nucleopolyhedrovirus ด้วย PCR-Based Typing
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