สืบค้นงานวิจัย
Development of competitive lateral flow immunoassay coupled with silver enhancement for simple and sensitive salivary cortisol detection
Apilux A. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Development of competitive lateral flow immunoassay coupled with silver enhancement for simple and sensitive salivary cortisol detection
ผู้แต่ง / หัวหน้าโครงการ (EN): Apilux A.
บทคัดย่อ (EN): Cortisol is known as a stress biomarker. The measurement of cortisol levels is an early warning indicator for health conditions and diagnosis of stress-related diseases. Herein, a lateral flow immunoassay using a gold nanoparticle label with a silver enhancement system was developed for the simple, sensitive and rapid detection of cortisol. The developed assay was based on a competitive platform of which cortisol-BSA conjugate was immobilized at the test zone to compete with an analyte. The quantitative analysis was performed using gold nanoparticles (AuNPs) as signal labeling. Sequentially, the silver enhancement solution was applied in order to enhance the sensitivity of the assay with the results easily seen by the naked eye. Using this system, the limit of detection (LOD) was found to be 0.5 ng/mL with a 3.6 fold more sensitive detection than without the enhancement system (LOD = 1.8 ng/mL). The salivary cortisol analysis was in the range of 0.5-150 ng/mL (R 2 = 0.9984), which is in the clinical acceptable range. For the semi-quantitative analysis, the intensity color of the results was analyzed using an image processing program. The proposed method was successfully applied to detect cortisol in saliva. In addition, the results from our method also complied with the ones of those obtained by using the commercial enzyme-linked immunosorbent assay (ELISA). This developed assay offers great promise for a non-invasive screening test of salivary cortisol. © 2018, Leibniz Research Centre for Working Environment and Human Factors. All rights reserved.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065076191&doi=10.17179%2fexcli2018-1824&partnerID=40&md5=7f9f5fc6b7806945176eb06dfd5ff629
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Silver enhancement
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Apilux A.. "Development of competitive lateral flow immunoassay coupled with silver enhancement for simple and sensitive salivary cortisol detection". ไม่ระบุหน่วยงาน. ม.ป.ป.

Apilux A.. "Development of competitive lateral flow immunoassay coupled with silver enhancement for simple and sensitive salivary cortisol detection". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Development of competitive lateral flow immunoassay coupled with silver enhancement for simple and sensitive salivary cortisol detection
Apilux A.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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