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Morphology, viability and phagocytic activity of blood and milk neutrophils during peripartum and early lactation of Murrah buffaloes (Buffalo Bulletin (Thailand),37(2))
A. K. Dang - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Morphology, viability and phagocytic activity of blood and milk neutrophils during peripartum and early lactation of Murrah buffaloes (Buffalo Bulletin (Thailand),37(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): A. K. Dang
หน่วยงานสังกัดผู้แต่ง (EN): Haryana (India). Dairy Cattle Physiology Division
บทคัดย่อ (EN): A comparative study of number, morphology, viability and activity in blood and milk neutrophils isolated from 10 Murrah buffaloes were carried out during different peripartum and early lactating days. Blood samples were collected from all buffaloes at days 30,15,7 before calving, on the day of calving and on days 7, 15, 30, 45 and 90 after calving. Colostrum and milk samples were collected on days 0, 5, 15, 30, 45, 60 and 90 days of early lactation. Blood total leucocyte counts (TLC), milk somatic cell counts (SCC), viability and morphology of neutrophils were measured microscopically. Blood and milk neutrophils were isolated and their in vitro phagocytic activity (PA) was estimated by Nitro Blue Tetrazolium assay. There were significant (P LT 0.05) increase in blood TLC and blood neutrophils percentage on the day of calving. Milk SCC and neutrophils in colostrums were significantly higher (P LT 0.05) than milk. On comparison of the viability of neutrophils isolated from blood, colostrum and milk, blood neutrophils viability was significantly (P LT 0.05) higher than both. Partum blood neutrophils viability was significantly (P LT 0.05) higher than other peripartum days. Similarly colostrum neutrophils had significantly (P LT 0.05) higher viability than its milk counterparts during different postpartum days. Colostrum neutrophils had a significantly (P LT 0.05) higher PA than milk neutrophils on different early lactation days and also from blood neutrophils on the day of calving. Blood neutrophils on different peripartum days had a more lobed and matured nucleus when compared with the morphology of blood neutrophils observed at calving, where they showed band shaped nucleus. Milk neutrophils had more lobed nucleus as compared to blood neutrophils. Scanning Electron Microscopy revealed more ruffled surface of blood than milk neutrophils on early lactation days. This study shows the changes occurring in the shape, viability and PA of blood neutrophils once they cross the blood mammary barrier to become milk neutrophils. Further understanding the functions of neutrophils around peripartum period and early lactation may help to develop more efficient strategies to combat immunosuppression around this period.
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ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/380918
คำสำคัญ (EN): India
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A. K. Dang. "Morphology, viability and phagocytic activity of blood and milk neutrophils during peripartum and early lactation of Murrah buffaloes (Buffalo Bulletin (Thailand),37(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.

A. K. Dang. "Morphology, viability and phagocytic activity of blood and milk neutrophils during peripartum and early lactation of Murrah buffaloes (Buffalo Bulletin (Thailand),37(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Morphology, viability and phagocytic activity of blood and milk neutrophils during peripartum and early lactation of Murrah buffaloes (Buffalo Bulletin (Thailand),37(2))
A. K. Dang
ไม่ระบุผู้เผยแพร่
ไม่ระบุวันที่เผยแพร่
Developing feeding module for increasing milk production in Murrah buffaloes (Bubalus bubalis) (Buffalo Bulletin (Thailand),37(1)) Hydrallantois in buffaloes (Buffalo Bulletin,37(3)) Surgical management of melanocytomas in buffaloes (Buffalo Bulletin (Thailand),37(2)) Comparative study on sperm morphology and morphometry of Holstein Friesian and Murrah buffalo bull (Buffalo Bulletin (Thailand),37(4)) Evaluation of milk fat as an alternative source of RNA from buffalo milk (Buffalo Bulletin (Thailand),37(2)) Comparative evaluation of functional activity of neutrophil in high and low yielding Murrah buffaloes during peripartum period (Buffalo Bulletin (Thailand),36(1)) Association of blood metabolites with reproductive disorders in postpartum Murrah buffaloes (Buffalo Bulletin (Thailand),35(4)) The reproductive performances of Murrah and swamp buffaloes in Thailand (Buffalo Bulletin,40(3)) Standardizing pregnancy rate of Indian Murrah buffaloes for higher milk yield (Buffalo Bulletin (Thailand),35(1)) Brachygnathia (parrot mouth) inferior calf in a Murrah buffalo (Buffalo Bulletin (Thailand),37(1))
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