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Identification of IL-1α gene from bovine peripheral blood mononuclear cells by polymerase chain reaction (Buffalo Bulletin (Thailand),36(2))
Pattnaik, B. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Identification of IL-1α gene from bovine peripheral blood mononuclear cells by polymerase chain reaction (Buffalo Bulletin (Thailand),36(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Pattnaik, B.
หน่วยงานสังกัดผู้แต่ง (EN): Uttarakhand (India)
บทคัดย่อ (EN): Interleukin-1 (IL-1) play its role in inflammation, fever and release of acute phase proteins by acting on major organs of the body. IL- 1α gene along with other cytokines compels Th1 and Th17 inflammatory responses. The present study is aimed at standardization of polymerase chain reaction for amplification of IL-1α gene from bovine peripheral blood mononuclear cells. Blood samples were collected in containers with 0.1% Ethylenediaminetetraacetic acid (EDTA) anticoagulant (Sigma, USA). Peripheral blood mononuclear cells (PBMCs) were isolated using commercially available Histopaque®-1077. Extracted total ribose nucleic acid (RNA) was utilized in reverse transcription to prepare complementary deoxyribose nucleic acid (cDNA). IL-1α gene specific primers were used to amlify partial nucleotide sequence of IL-1α. The cDNA was employed in polymerase chain reaction (PCR). The resulting IL-1α DNA product (124 bp) was identified on agar gel electrophoresis and documented.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/368607
คำสำคัญ (EN): India
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Identification of IL-1α gene from bovine peripheral blood mononuclear cells by polymerase chain reaction (Buffalo Bulletin (Thailand),36(2))
Pattnaik, B.
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Oesophageal obstruction in a pregnant buffalo (Buffalo Bulletin (Thailand),36(4)) Infectious bovine keratoconjunctivitis in a buffalo calf (Buffalo Bulletin (Thailand),37(1)) Karyotype of Mazani water buffalo from Iran (Buffalo Bulletin (Thailand),36(1)) Influence of Zn supplementation (organic and inorganic) on blood testosterone profile of Murrah buffalo bulls (Buffalo Bulletin (Thailand),36(3)) Dystocia due to foetal ascites in a Murrah buffalo (Buffalo Bulletin (Thailand),36(3)) Congenital anomalies of the uterus in riverine buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(4)) Management of rare case of Emamectin benzoate poisoning in a buffalo (Buffalo Bulletin (Thailand),36(4)) Isolation of hippuric acid from buffalo urine and its antioxidant activity (Buffalo Bulletin (Thailand),36(3)) Cryopreservation of buffalo (Bubalus bubalis) semen-limitations and expectations (Buffalo Bulletin (Thailand),36(1)) β(1,4)-Galactosyltransferase-I gene polymorphisms in Pakistani Nili Ravi buffalo (Buffalo Bulletin (Thailand),35(3))
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