สืบค้นงานวิจัย
Comparison of parasitological, serological and molecular diagnostic tests for detection of subclinical Trypanosomosis in two organised dairy herds (Buffalo Bulletin,41(2))
Parveen Goel - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Comparison of parasitological, serological and molecular diagnostic tests for detection of subclinical Trypanosomosis in two organised dairy herds (Buffalo Bulletin,41(2))
ผู้แต่ง / หัวหน้าโครงการ (EN): Parveen Goel
หน่วยงานสังกัดผู้แต่ง (EN): Haryana (India)
บทคัดย่อ (EN): Trypanosomosis also known as 'Surra' caused by Trypanosoma evansi is of significant economic importance in livestock production. Diagnosis of subclinical infection and carrier state of disease is far difficult. Therefore, the present study was conducted to compare parasitological, serological and molecular diagnostic tests for detecting subclinical trypanosomosis in two dairy herds of Murrah buffalo (n=27) and crossbred cattle (n=22) in Hisar district of Haryana. Peripheral blood samples collected from all the animals were subjected to blood smear examination and DNA isolation for molecular detection by PCR, and like-wise serum samples were collected and subjected to latex agglutination test (TE-LAT) for T. evansi antigen detection. PCR products from positive samples were purified and sequenced for confirmation of T. evansi DNA. None of the animals in the two herds was found positive for T. evansi parasites by microscopic examination of thick blood smears. In buffalo farm, 29.62 percent samples each were found moderately and weak positive by TE-LAT, whereas PCR assay test yielded 40.74 percent samples positive for T. evansi DNA. In cattle farm, TE-LAT showed 18.18 percent, 31.89 percent and 9.09 percent samples to be strong, moderate and weak positive, whereas 50 percent samples were found PCR-positive for T. evansi DNA. Agreement among TE-LAT and PCR assay, as expressed by the kappa value, was found to be poor. To conclude, each test has its own merits and limitations. While TE-LAT is suitable for screening purpose, PCR assay confirms parasite in blood. For clinical diagnosis, results of laboratory diagnostic tests must, however, be correlated clinically before initiating the therapy.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/418123
คำสำคัญ (EN): PCR assay
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Parveen Goel. "Comparison of parasitological, serological and molecular diagnostic tests for detection of subclinical Trypanosomosis in two organised dairy herds (Buffalo Bulletin,41(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Parveen Goel. "Comparison of parasitological, serological and molecular diagnostic tests for detection of subclinical Trypanosomosis in two organised dairy herds (Buffalo Bulletin,41(2))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Comparison of parasitological, serological and molecular diagnostic tests for detection of subclinical Trypanosomosis in two organised dairy herds (Buffalo Bulletin,41(2))
Parveen Goel
ไม่ระบุผู้เผยแพร่
ไม่ระบุวันที่เผยแพร่
Comparison of different diagnostic tests for the detection of subclinical mastitis in buffaloes (Buffalo Bulletin,40(4)) Molecular characterization of buffalo alpha s1-casein gene (Buffalo Bulletin,41(3)) Isolation and molecular detection of biofilm producing and multiple drug resistant Enterococcus faecalis from the buffalo meat (Buffalo Bulletin,41(3)) Efficacy of antioxidant in the therapy of subclinical mastitis in buffaloes (Buffalo Bulletin,41(1)) Hydrallantois in a graded Murrah buffalo - A case report (Buffalo Bulletin,41(2)) Fatty acid profile of Murrah buffalo milk fat (Buffalo Bulletin,41(1)) Dystocia due to Dicephalic dipus dibrachius monster in a Murrah buffalo (Buffalo Bulletin,41(3)) Epidemiological, clinico-diagnostic and therapeutic aspects of bovine respiratory disease in Indian buffaloes (Buffalo Bulletin,41(4)) Dystocia due to bull dog calf in a Murrah buffalo heifer (Buffalo Bulletin,41(1)) Constraints of buffalo farming in Kallakurichi district of Tamil Nadu, India (Buffalo Bulletin,41(3))
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