สืบค้นงานวิจัย
CHARACTERIZATION OF LATEX-CLEARING-PROTEIN FROM A RUBBER-DEGRADING BACTERIA ISOLATED IN VIETNAM
Dao Viet Linh - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): CHARACTERIZATION OF LATEX-CLEARING-PROTEIN FROM A RUBBER-DEGRADING BACTERIA ISOLATED IN VIETNAM
ผู้แต่ง / หัวหน้าโครงการ (EN): Dao Viet Linh
บทคัดย่อ (EN): Characterization of bacterial rubber degrading enzyme genes will lead to the use of recombinant enzymes and application of rubber degradation products for new carboxylated cross- linking polymers and new processing system for surface fabrication. We are focusing on a latex clearing protein (lcp) from the Nocardia sp. NVL3 which was isolated from wastes of a rubber processing factory in Thanh Hoa province, Vietnam. Thus, an lcp in strain NVL3 was cloned in a cloning vector and was heterologously expressed in E. coli BL21 (DE3) resulting a recombinant BL21::pEHIcpH. In this study, we are successful to purify His-tagged Lcp from soluble fraction of BL21::pEHIcpH cells and separated it in a SDS - Polyacrylamide gel. In order to analyse peptide mass fingerprint, in-gel 47.3 kDa protein then was purified and digested with Trypsin. As the results of Matrix-assisted laser desorption/ionization - Time of flight mass spectrometer (MALDI-TOF/MS), all peptides of Lcp were separated in sequence and computed for mass. To confirm the enzymatic activity, we carried out the reaction in vitro by using 0.4 mg of purified His-tagged Lcp with in-flask coated synthetic poly (cis-1,4-isoprene) and flask samples were incubated for 24 hours at 30◦C or at 37◦C.Gel permeation choromatography (GPC) analysis of those samples show the degeneration of a peak derived from poly (cis-1,4-isoprene) and the arising of the another peak at higher retension time. By that, we strongly comment Lcp itself cleaves rubber polymer to lower molecular weight products. In contrast, no rubber degradation lead by extracellular proteins of BL21::pEHLcpH culture can be observed. Consistently, Schiffs staining in a paper disk assay revealed that purified Lcp and soluble protein fraction from BL21::pEHLcpH cells can accumulated Aldehydes persistently on the rubber plate. Thus, our study indicated Lcp is involved in rubber degradation of strain NVL3 by cleavage of rubber polymer into lower-molecular-weight aldehyde-containing compounds. This protein can be purified highly for detail enzymatic characterization and further applications on rubber processing can be acquired.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): enzymes
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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การอ้างอิง

Dao Viet Linh. "CHARACTERIZATION OF LATEX-CLEARING-PROTEIN FROM A RUBBER-DEGRADING BACTERIA ISOLATED IN VIETNAM". ไม่ระบุหน่วยงาน. ม.ป.ป.

Dao Viet Linh. "CHARACTERIZATION OF LATEX-CLEARING-PROTEIN FROM A RUBBER-DEGRADING BACTERIA ISOLATED IN VIETNAM". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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CHARACTERIZATION OF LATEX-CLEARING-PROTEIN FROM A RUBBER-DEGRADING BACTERIA ISOLATED IN VIETNAM
Dao Viet Linh
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Advances on biodiversity of natural rubber biosynthesis and regulations on natural rubber biosynthesis Technological and processing properties of natural rubber layered silicate-nanocomposites by melt intercalation process SUPPORTS OF THE VIETNAM RUBBER ASSOCIATION FOR RUBBER INDUSTRY Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 The 2011-2015 Rubber Planting Recommendation In Vietnam CHARACTERIZATION OF COMMERCIAL NATURAL RUBBER THROUGH NMR SPECTROSCOPY VIETNAM RUBBER PLANTING RECOMMENDATION 2016-2020 CHARACTERIZATION AND TREATMENT OF SLUDGE FROM THE RUBBER PROCESSING INDUSTRY WITH LOCALLY SOURCED COAGULANTS (CHITOSAN AND JATROPHA GOSSYPIFOLIA STEM LATEX) Visco-Elastic Behaviour and Reinforcement of Montmorillonite Clay Filled Natural Rubber Latex Nano-Crepe Rubber GREEN RUBBER RECOVERY AGENT FOR SKIM LATEX (GRASS 1)
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