สืบค้นงานวิจัย
Detection of an Unknown Trypanosoma DNA in a Phlebotomus stantoni (Diptera: Psychodidae) Collected From Southern Thailand and Records of New Sand Flies With Reinstatement of Sergentomyia hivernus Raynal & Gaschen, 1935 (Diptera: Psychodidae)
Phumee - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Detection of an Unknown Trypanosoma DNA in a Phlebotomus stantoni (Diptera: Psychodidae) Collected From Southern Thailand and Records of New Sand Flies With Reinstatement of Sergentomyia hivernus Raynal & Gaschen, 1935 (Diptera: Psychodidae)
ผู้แต่ง / หัวหน้าโครงการ (EN): Phumee
บทคัดย่อ (EN): Although female sand flies are best known as the vectors of Leishmania parasites and viruses, several previous reports have demonstrated that these insects can also act as vectors for the trypanosomes of bats, lizards, and snakes. In this report, we created an inventory of Phlebotomine sand flies from southern Thailand. A novel trypanosome was found in a specimen of Phlebotomus stantoni, and two sand fly species newly recorded in the country, Sergentomyia khawi and Sergentomyia hivernus, were described. PCR primer pairs specific for the internal transcribed spacer 1 (ITS1) and the small subunit ribosomal DNA (SSU rDNA) gene of trypanosomatids were used to demonstrate the presence of the parasite in the sand fly. In addition, the Cytochrome b (CytB) gene was used to identify the sand fly species. Among the 45 samples of the sand fly that were collected, seven samples were Ph. stantoni sand flies and a single sample was positive for Trypanosoma sp. through PCR analysis. This study represents the first detection of Trypanosoma sp. in a sand fly from Thailand. The ITS1 and SSU rDNA sequences indicated that this specimen is suspected to be a novel Trypanosoma species. Further studies of this suspected new Trypanosoma species, including its vertebrate hosts and pathogenic potential, are therefore necessary.
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ภาษา (EN): en
เอกสารแนบ (EN): http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=498
เผยแพร่โดย (EN): 1 Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand 2 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 3 Faculty of Medical Technology, Prince of Songkla University, Songkhla, Thailand 4 The Office of Disease Prevention and Control 12, Songkhla, Thailand 5 Université de Reims Champagne Ardenne, ANSES, SFR Cap santé, EA 4688 - USC "transmission vectorielle et épidemiosurveillance de maladies parasitaires (VECPAR)", Reims, France 6 Université Pierre et Marie Curie-Paris 6, CHU Pitié-Salpêtrière, AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Service Parasitologie-Mycologie, Paris, France 7 Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand 8 Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand.
คำสำคัญ (EN): ITS1; PCR; SSU rDNA; Trypanosoma sp.; sand fly
เจ้าของลิขสิทธิ์ (EN): National Institute of Health, Department of Medical Science
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Detection of an Unknown Trypanosoma DNA in a Phlebotomus stantoni (Diptera: Psychodidae) Collected From Southern Thailand and Records of New Sand Flies With Reinstatement of Sergentomyia hivernus Raynal & Gaschen, 1935 (Diptera: Psychodidae)
Phumee
1 Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand 2 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand 3 Faculty of Medical Technology, Prince of Songkla University, Songkhla, Thailand 4 The Office of Disease Prevention and Control 12, Songkhla, Thailand 5 Université de Reims Champagne Ardenne, ANSES, SFR Cap santé, EA 4688 - USC "transmission vectorielle et épidemiosurveillance de maladies parasitaires (VECPAR)", Reims, France 6 Université Pierre et Marie Curie-Paris 6, CHU Pitié-Salpêtrière, AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Service Parasitologie-Mycologie, Paris, France 7 Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand 8 Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand.
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