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Interferon-gamma messenger RNA level in peripheral blood mononuclear cells of naive bovines after foot and mouth disease virus vaccination and challenge (Buffalo Bulletin,38(3))
B. Pattnaik - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Interferon-gamma messenger RNA level in peripheral blood mononuclear cells of naive bovines after foot and mouth disease virus vaccination and challenge (Buffalo Bulletin,38(3))
ผู้แต่ง / หัวหน้าโครงการ (EN): B. Pattnaik
หน่วยงานสังกัดผู้แต่ง (EN): Uttarakhand (India)
บทคัดย่อ (EN): The study evaluated the antibody response and interferon-gamma (IFN-gamma) messenger ribonucleic acid (mRNA) expression levels in naive bovine calves from a Foot-and-Mouth Disease virus (FMDV) potency test. Peripheral blood mononuclear cells (PBMCs) were separated from blood samples and total RNA was extracted. Two step quantitative (Real time-polymerase chain reaction (RT-PCR) method was used for measurement of IFN-gamma specific mRNA. Plasma samples were screened to detect anti-non structural proteins (NSP) antibodies for FMDV in 3AB NSP antibody enzyme linked immunosorbent assay (ELISA). Plasma samples were found free for anti- NSP antibodies for FMDV in vaccinated bovine calves as evaluated in 3AB NSP antibody ELISA test. Increase in mRNA expression level of IFN-gamma after 15 days post vaccination (DPV) was found unequivocally in all five vaccinated calves (more than two-fold increase in 4 out of 5 calves). Liquid phase blocking ELISA (LPBE) test results also revealed highest humoral antibody response on 15 DPV. Monovalent FMDV vaccination elicited highest antibody titre on 15 DPV in correlation with elevated IFN-gamma mRNA level. Humoral and cellular immunity both play important role in protection against FMDV. The study will help in further understanding the role played by both the humoral and cellular immune response against FMDV.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/390587
คำสำคัญ (EN): India
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B. Pattnaik. "Interferon-gamma messenger RNA level in peripheral blood mononuclear cells of naive bovines after foot and mouth disease virus vaccination and challenge (Buffalo Bulletin,38(3))". ไม่ระบุหน่วยงาน. ม.ป.ป.

B. Pattnaik. "Interferon-gamma messenger RNA level in peripheral blood mononuclear cells of naive bovines after foot and mouth disease virus vaccination and challenge (Buffalo Bulletin,38(3))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Interferon-gamma messenger RNA level in peripheral blood mononuclear cells of naive bovines after foot and mouth disease virus vaccination and challenge (Buffalo Bulletin,38(3))
B. Pattnaik
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Toll like receptor 7 messenger RNA expression levels in dairy bovines in an outbreak of Foot-and-Mouth disease (Buffalo Bulletin (Thailand),36(3)) Detection of foot and mouth disease virus shedding in milk of apparently healthy buffaloes and cattle of Punjab, Pakistan (Buffalo Bulletin,38(2)) Analysis of the stress and haematological changes after Foot-and-mouth disease vaccination in buffaloes (Buffalo Bulletin,39(2)) Identification of IL-1α gene from bovine peripheral blood mononuclear cells by polymerase chain reaction (Buffalo Bulletin (Thailand),36(2)) Investigations of foot and mouth disease outbreaks in different districts of Punjab, Pakistan (Buffalo Bulletin (Thailand),37(1)) Study on lead level in blood and tissue samples of buffaloes (Buffalo Bulletin,40(2)) Onion toxicosis in buffaloes (Buffalo Bulletin,38(2)) Ultrstructural study on efferent ductule of buffalo foetii (Buffalo Bulletin,38(3)) Rectal prolapse in crossbred buffalo (Bubalus bubalis) calf (Buffalo Bulletin,38(1)) In silico characterization of Mx2 protein of buffalo (Bubalus bubalis) (Buffalo Bulletin,38(4))
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