สืบค้นงานวิจัย
Recombinase polymerase amplification assay for rapid diagnostics of dengue infection
Abd El Wahed A. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Recombinase polymerase amplification assay for rapid diagnostics of dengue infection
ผู้แต่ง / หัวหน้าโครงการ (EN): Abd El Wahed A.
บทคัดย่อ (EN): Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for molecular detection of the dengue virus (DENV). Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4. Methodology/Principal Findings: Using two quantitative RNA molecular standards, the analytical sensitivity of a RT-RPA targeting the 3′non-translated region of DENV1-4 was found to range from 14 (DENV4) to 241 (DENV1-3) RNA molecules detected. The assay was specific and did not cross detect other Flaviviruses. The RT-RPA assay was tested in a mobile laboratory combining magnetic-bead based total nucleic acid extraction and a portable detection device in Kedougou (Senegal) and in Bangkok (Thailand). In Kedougou, the RT-RPA was operated at an ambient temperature of 38°C with auxiliary electricity tapped from a motor vehicle and yielded a clinical sensitivity and specificity of 98% (n=31) and 100% (n=23), respectively. While in the field trial in Bangkok, the clinical sensitivity and specificity were 72% (n=90) and 100% (n=41), respectively. Conclusions/Significance: During the first 5 days of infection, the developed DENV1-4 RT-RPA assays constitute a suitable accurate and rapid assay for DENV diagnosis. Moreover, the use of a portable fluorescence-reading device broadens its application potential to the point-of-care for outbreak investigations. © 2015 Abd El Wahed et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84937013070&doi=10.1371%2fjournal.pone.0129682&partnerID=40&md5=97ad0734bfe7e674d18487a880840fa5
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Thailand
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Abd El Wahed A.. "Recombinase polymerase amplification assay for rapid diagnostics of dengue infection". ไม่ระบุหน่วยงาน. ม.ป.ป.

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Recombinase polymerase amplification assay for rapid diagnostics of dengue infection
Abd El Wahed A.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Relationships between dengue virus infection in mosquito vector, (Aedes aegypti), dengue cases and weather conditions in Samut Sakhon Province, Thailand Induction of defensin response to dengue infection in Aedes aegypti Impact of glucose-6-phosphate dehydrogenase deficiency on dengue infection in Myanmar children Double Dengue Serotypes in Asymptomatic Populations Living in An Area of Thailand Endemic for Dengue Hemorrhagic Fever Temperature of a Dengue Rapid Diagnostic Test under Tropical Climatic Conditions: A Follow Up Study Identification of changes in dendritic cell subsets that correlate with disease severity in dengue infection Streptococcus agalactiae infection kills red tilapia with chronic Francisella noatunensis infection more rapidly than the fish without the infection First evidence of dengue infection in domestic dogs living in different ecological settings in Thailand Microparticles provide a novel biomarker to predict severe clinical outcomes of dengue virus infection Potent neutralizing human monoclonal antibodies preferentially target mature dengue virus particles: Implication for novel strategy for dengue vaccine
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