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In vitro generation of dendritic cell - breast cancer cell hybrids for induction of cellular immunity
Supaporn Suparak - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): In vitro generation of dendritic cell - breast cancer cell hybrids for induction of cellular immunity
ผู้แต่ง / หัวหน้าโครงการ (EN): Supaporn Suparak
บทคัดย่อ (EN): Dendritic cells (DCs) of cancer patients are unable to effectively process and present tumor associated antigens to immune cells, resulting in a spread of cancer cells. Our study was to enhance in vitro efficacy of DCs to further induce activities of T lymphocytes and Natural Killer (NK) cells for inhibition/ reduction of a growth of a breast cancer cell line (MCF-7). Human monocytes, derived from buffy coat of six normal blood donors, were positively selected and generated DCs by culturing with recombinant GM-CSF and IL-4 for 6 days. Human monocyte-derived DCs were determined by a decrease in a level of CD14 and an increase in CD11c and HLA-DR levels by flow cytometry. The DCs were further pulsed with breast cancer cell lines (MCF-7) to generate DCs - MCF-7 tumor fusion cells with a mean success rate at 34.67%. The DCs - MCF-7 fusion cells were able to stimulate T cell proliferation as well as to enhance NK cells activities to kill MCF-7 from an average of 22.45% to 72.00% and 22.91% to 65.69%, respectively. Our study demonstrated a model for in vitro generation of effective human monocyte derived DCs – tumor fusion cells and a possibility to further develop alternative method to treat cancer patients.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=554
เผยแพร่โดย (EN): 1 National Institute of Health 2 Medical Sciences Technical Office, Department of Medical Sciences, Tiwanond Road, Nonthaburi 11000, Thailand.
คำสำคัญ (EN): Dendritic cells; T lymphocyte; NK cell; MCF-7; breast cancer
เจ้าของลิขสิทธิ์ (EN): National Institute of Health, Department of Medical Science
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In vitro generation of dendritic cell - breast cancer cell hybrids for induction of cellular immunity
Supaporn Suparak
1 National Institute of Health 2 Medical Sciences Technical Office, Department of Medical Sciences, Tiwanond Road, Nonthaburi 11000, Thailand.
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