สืบค้นงานวิจัย
Colorimetric method of loop-mediated isothermal amplification with the pre-addition of calcein for detecting flavobacterium columnare and its assessment in tilapia farms
Suebsing R. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Colorimetric method of loop-mediated isothermal amplification with the pre-addition of calcein for detecting flavobacterium columnare and its assessment in tilapia farms
ผู้แต่ง / หัวหน้าโครงการ (EN): Suebsing R.
บทคัดย่อ (EN): Flavobacterium column are, the causative agent of columnar is disease in fish, affects many economically important freshwater fish species. A colorimetric method of loop-mediated isothermal amplification with the pre-addition of calcein(LAMP–calcein) was developed and used to detect the presence of F.column are infarmed tilapia (NileTilapia Oreochromis niloticus and red tilapia [Nile Tilapia × Mozambique Tilapia O. mossambicus]) and rearing water. The detection method, based on a change in color from orange to green, could be performed within 45 min at 63◦C. The method was highly specific, as it had no cross-detections with 14 other bacterial species, including other fish pathogens and two Flavobacterium species. The method has a minimum detection limit of 2.2 × 102 F. columnare CFU; thus, it is about 10 times more sensitive than conventional PCR. With this method, F. columnare was detected in gonad, gill, and blood samples from apparently healthy tilapia broodstock as well as in samples of fertilized eggs, newly hatched fry, and rearing water. The bacteria isolated from the blood were further characterized biochemically and found to be phenotypically identical to F. columnare. The amplified products from the LAMP–calcein method had 97% homology with the DNA sequence of F. columnare. © American Fisheries Society 2015
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84921263135&doi=10.1080%2f08997659.2014.966212&partnerID=40&md5=68c39dfdd3b51ad1174d7a65678ecdcd
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Tilapia
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Colorimetric method of loop-mediated isothermal amplification with the pre-addition of calcein for detecting flavobacterium columnare and its assessment in tilapia farms
Suebsing R.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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