สืบค้นงานวิจัย
Low levels of antibody-dependent enhancement in vitro using viruses and plasma from dengue patients
Chaichana P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Low levels of antibody-dependent enhancement in vitro using viruses and plasma from dengue patients
ผู้แต่ง / หัวหน้าโครงการ (EN): Chaichana P.
บทคัดย่อ (EN): The majority of dengue patients infected with any serotype of dengue virus (DENV) are asymptomatic, but the remainder may develop a wide spectrum of clinical symptoms, ranging from mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF). Severe cases occur more often in patients who experience a secondary infection with a different virus serotype. A phenomenon called antibody-dependent enhancement (ADE) has been proposed to explain the onset of these severe cases, but the exact mechanism of ADE remains unclear. Methodology/Principal Finding: Virus neutralization and ADE assays were performed using ultracentrifugation supernatants of acute-phase sera from patients with secondary infections or human monoclonal antibodies (HuMAbs) as anti-DENV antibodies. Virus sources included infectious serum-derived viruses from the ultracentrifugation precipitates, laboratory-culture adapted DENV, or recombinant DENVs derived from patient sera. In contrast to the high levels of ADE observed with laboratory virus strains, low ADE was observed with autologous patient-derived viruses, when patient sera were used to provide the antibody component in the ADE assays. Similar results were obtained using samples from DF and DHF patients. Recombinant-viruses derived from DHF patients showed only minor differences in neutralization and ADE activity in the presence of HuMAbs or plasma derived from the same DHF patient. Conclusion/Significance: Serum or plasma taken from patients during the acute phase of a secondary infection showed high levels of ADE, but no neutralization activity, when assayed in the presence of laboratory-adapted virus strains. By contrast, serum or plasma from the same patient showed high levels of neutralization activity but failed to induce significant ADE when the assays were performed with autologous virus. These results demonstrate the significance of the virus source when measuring ADE. They also suggest that repeated passage of DENV in cell culture has endowed it with the capacity to induce high levels of ADE. © 2014 Chaichana et al.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84898658030&doi=10.1371%2fjournal.pone.0092173&partnerID=40&md5=3b71675dabe8edb60316878cf180078a
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Vero Cells
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Chaichana P.. "Low levels of antibody-dependent enhancement in vitro using viruses and plasma from dengue patients". ไม่ระบุหน่วยงาน. ม.ป.ป.

Chaichana P.. "Low levels of antibody-dependent enhancement in vitro using viruses and plasma from dengue patients". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Low levels of antibody-dependent enhancement in vitro using viruses and plasma from dengue patients
Chaichana P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Inverse association of plasma IgG antibody to Aggregatibacter actinomycetemcomitans and high C-reactive protein levels in patients with metabolic syndrome and periodontitis Dengue forecasting model using sarima model to predict the incidence of dengue in Thailand Characterization of human CD8 T cell responses in dengue virus-infected patients from India Management and outcomes of severe dengue patients presenting with sepsis in a tropical country Zika Incidence among Patients with Suspected Dengue Fever (Preliminary Report) Dengue serotype and clinical severity among pediatric patients : a decade review Potent neutralizing human monoclonal antibodies preferentially target mature dengue virus particles: Implication for novel strategy for dengue vaccine Argon Plasma Treatment of Tapioca Starch Using a Semi-continuous Downer Reactor Pregnancy enhancement using ovsynch protocol in Murrah buffaloes (Buffalo Bulletin,37(4)) Spatial variations in dengue transmission in schools in Thailand
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair