คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Nuttapon Jirakittiwut

เผยแพร่โดย

1 Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 2 Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 3 The Chemical Approaches for Food Applications Research Group, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 4 Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Phuttamonthon 4 Rd., Salaya, Nakhon Pathom, 73170, Thailand 5 Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 6 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, 11000, Thailand 7 Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 8 The Chemical Approaches for Food Applications Research Group, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand

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งานวิจัยที่แนะนำ External Quality Assessment Scheme for HIV-1 Drug Resistance Genotyping in Thailand Sensitive visual detection of AHPND bacteria using loop-mediated isothermal amplification combined with DNAFunctionalized gold nanoparticles as probes การประกันคุณภาพทางห้องปฏิบัติการด้านโมเลกุลธาลัสซีเมีย Proficiency Testing Programme in Molecular Thalassemia detection Pesticide analysis using nanoceria-coated paper-based devices as a detection platform Successive detection of benzoic acid and total parabens in foodstuffs using mercaptosuccinic acid capped cadmium telluride quantum dots ผลของแบคทีเรียที่ผลิตสาร Indole-3-Acetic Acid (IAA) ต่อการเจริญเติบโตและ ปริมาณธาตุอาหารของพริกขี้หนู GENETIC TRANSFORMATION OF HEVEA BRASILIENSIS BY USING A NON-DESTRUCTIVE VISUAL MARKER (GFP) Detection of Mycobacterium tuberculosis and Its Rifampicin Resistance by Automated Nucleic Acid Amplification Test Twelve-week protocatechuic acid administration improves insulin-induced and insulin-like growth factor-1-induced vasorelaxation and antioxidant activities in aging spontaneously hypertensive rats Hydrolysis of delignified sugarcane bagasse using hydrothermal technique catalyzed by carbonaceous acid catalysts

คัดลอก URL

ชื่อเรื่อง (EN):	Visual genotyping of thalassemia by using pyrrolidinyl peptide nucleic acid probes immobilized on carboxymethylcellulose-modified paper and enzyme-induced pigmentation
ผู้แต่ง / หัวหน้าโครงการ (EN):	Nuttapon Jirakittiwut
บทคัดย่อ (EN):	A simple probe pair was designed for the detection of hemoglobin E (HbE) genotype, a single-point mutation that leads to abnormal red blood cells commonly found in South East Asia. The key to differentiation is the use of a conformationally constrained peptide nucleic acid (PNA) that was immobilized on carboxymethylcellulose-modified paper. This was then used for target DNA binding and visualization by an enzyme-catalyzed pigmentation. The biotinylated target DNA bound to the immobilized probe was visually detected via alkaline phosphatase-linked streptavidin. This enzyme conjugate catalyzed the dephosphorylation of the substrate 5-bromo-4-chloro-3-indolyl phosphate, leading to a series of reactions that generate an intense, dark blue pigment. The test was validated with 100 DNA samples, which shows good discrimination among different genotypes (normal, HbE, and heterozygous) with 100% accuracy when optimal conditions of analysis were applied. The method does not require temperature control and can be performed at ambient temperature. This is an attractive feature for diagnosis in primary care, which accounts for a large part of affected population. Graphical abstract Schematic representation of a paper-based sensor for the detection of the gene Hemoglobin E. The interaction between an immobilized peptide nucleic acid and a DNA target leads to enzymatic pigmentation, allowing simple visual readout with up to 100% accuracy.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=571
เผยแพร่โดย (EN):	1 Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 2 Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 3 The Chemical Approaches for Food Applications Research Group, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 4 Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Phuttamonthon 4 Rd., Salaya, Nakhon Pathom, 73170, Thailand 5 Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 6 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, 11000, Thailand 7 Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand 8 The Chemical Approaches for Food Applications Research Group, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, Bangkok, 10330, Thailand
คำสำคัญ (EN):	Thalassemia
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science