สืบค้นงานวิจัย
Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray
Utsumi Y. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray
ผู้แต่ง / หัวหน้าโครงการ (EN): Utsumi Y.
บทคัดย่อ (EN): Cassava anthracnose disease (CAD), caused by the fungus Colletotrichumgloeosporioides f. sp. Manihotis, is a serious disease of cassava (Manihot esculenta) worldwide. In this study, we established a cassava oligonucleotide-DNA microarray representing 59,079 probes corresponding to approximately 30,000 genes based on original expressed sequence tags and RNA-seq information from cassava, and applied it to investigate the molecular mechanisms of resistance to fungal infection using two cassava cultivars, Huay Bong 60 (HB60, resistant to CAD) and Hanatee (HN, sensitive to CAD). Based on quantitative real-time reverse transcription PCR and expression profiling by the microarray, we showed that the expressions of various plant defense-related genes, such as pathogenesis-related (PR) genes, cell wall-related genes, detoxification enzyme, genes related to the response to bacterium, mitogen-activated protein kinase (MAPK), genes related to salicylic acid, jasmonic acid and ethylene pathways were higher in HB60 compared with HN. Our results indicated that the induction of PR genes in HB60 by fungal infection and the higher expressions of defense response-related genes in HB60 compared with HN are likely responsible for the fungal resistance in HB60. We also showed that the use of our cassava oligo microarray could improve our understanding of cassava molecular mechanisms related to environmental responses and development, and advance the molecular breeding of useful cassava plants. © 2016, The Botanical Society of Japan and Springer Japan.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84965069051&doi=10.1007%2fs10265-016-0828-x&partnerID=40&md5=0d11a58b1ef13d8ce6062ea29419fd66
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Up-Regulation
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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การอ้างอิง

Utsumi Y.. "Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray". ไม่ระบุหน่วยงาน. ม.ป.ป.

Utsumi Y.. "Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray
Utsumi Y.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Isolation and Characterization of Microsatellite Loci and Genetic Diversity in Cassava (Manihot esculenta Crantz) Involvement of miR164- and miR167-mediated target gene expressions in responses to water deficit in cassava Identification of Differentially Expressed Proteins in Cassava Infected with Colletotrichum gloeosporioides f. sp. manihotis Genome-wide analysis reveals phytohormone action during cassava storage root initiation Quantitative trait loci and candidate genes associated with starch pasting viscosity characteristics in cassava (Manihot esculenta Crantz) Genetic Linkage Map of Cassava (Manihot esculenta Crantz) Based on Rubber Tree and Cassava Simple Sequence Repeat Markers Identifying Pathogenicity Genes in the Rubber Tree Anthracnose Fungus Colletotrichum gloeosporioides through Random Insertional Mutagenesis Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples Quantitative trait loci underlying root yield and starch content in an F1 derived cassava population (Manihot esculenta Crantz) In vitro assessment of Ag-TiO 2 /polyurethane nanocomposites for infection control using response surface methodology
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