สืบค้นงานวิจัย
Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen
Buranaamnuay K. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen
ผู้แต่ง / หัวหน้าโครงการ (EN): Buranaamnuay K.
บทคัดย่อ (EN): The quality of cat epididymal sperm cryopreserved and stored by four methods was assessed. Epididymal sperm were suspended in Tris-glucose-citrate egg yolk extender, loaded in 0.25 mL straws and then cryopreserved. The samples in a standard protocol (LN) were cryopreserved and stored in liquid nitrogen (LN2). The sperm straws in the LN-Fr-LN group were cryopreserved in LN2 and stored in a −75 °C freezer; the straws were returned to LN2 prior to thawing. The loaded straws in the Fr group were transferred directly from 4 °C to the freezer and maintained in the freezer until thawing. The Fr-LN samples were cryopreserved and stored in the freezer and were introduced into LN2 before thawing. The sperm thawing was conducted on days 30, 60, 90 and 120 of cryopreservation. The sperm motility, viability, membrane integrity and acrosome integrity were evaluated at 15 and 180 min after thawing. The quality of post-thaw sperm in all three modified protocols was comparable (P > 0.05) and did not differ from that in the standard protocol except the membrane integrity of the 60 days stored samples evaluated at 15 min after thawing, which was significantly higher for the LN-Fr-LN than the Fr-LN groups (P = 0.04). The length of cryopreservation time had no effect (P > 0.05) on the sperm parameters assessed at 15 min after thawing. The sperm motility was significantly greater (P = 0.01 to P = 0.02) for the 15 min than the 180 min incubation. In conclusion, cat epididymal sperm could alternatively be cryopreserved and/or stored by using the −75 °C freezer for 120 days. To use, the cryopreserved sperm in the freezer could be thawed immediately or after being transferred to LN2. This was useful for the application of the −75 °C cryopreserved sperm in remote areas. © 2018 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042090716&doi=10.1016%2fj.anireprosci.2018.02.008&partnerID=40&md5=4e936c84694dc67b87e30f28b87b722f
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Time Factors
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Buranaamnuay K.. "Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen". ไม่ระบุหน่วยงาน. ม.ป.ป.

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Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen
Buranaamnuay K.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Determination of appropriate cryopreservation protocols for epididymal cat spermatozoa Effect of extracellular adenosine 5'-triphosphate on cryopreserved epididymal cat sperm intracellular ATP concentration, sperm quality, and in vitro fertilizing ability Protocols for sperm cryopreservation in the domestic cat: A review Preliminary study on effects of bovine frozen semen storage using a liquid nitrogen-independent method on the quality of post-thaw spermatozoa Treatment of a slaughterhouse wastewater using sequencing batch reactors at a shortened operating cycle L-carnitine supplemented extender improves cryopreserved-thawed cat epididymal sperm motility Changes of fatty acids in phosphatidylcholine on sperm membrane during Macrobrachium rosenbergii sperm transit through spermatic duct and lipid analysis in spermatic vesicles Hevea B Serum Proteome Profiling Using Liquid Chromatography-Mass Spectrometry Cryopreservation of Arundina graminifolia (D. Don) hochr. Seeds using D cryo-plate method NEW MIXTURE OF CATALYST TO REPLACE OF SELENIUM IN DETERMINATION OF NITROGEN USING KJELDAHL METHOD
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