สืบค้นงานวิจัย
Identification of laticifer-specific genes and their promoter regions from a natural rubber producing plant Hevea brasiliensis
Aoki Y. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Identification of laticifer-specific genes and their promoter regions from a natural rubber producing plant Hevea brasiliensis
ผู้แต่ง / หัวหน้าโครงการ (EN): Aoki Y.
บทคัดย่อ (EN): Latex, the milky cytoplasm of highly differentiated cells called laticifers, from Hevea brasiliensis is a key source of commercial natural rubber production. One way to enhance natural rubber production would be to express genes involved in natural rubber biosynthesis by a laticifer-specific overexpression system. As a first step to identify promoters which could regulate the laticifer-specific expression, we identified random clones from a cDNA library of H. brasiliensis latex, resulting in 4325 expressed sequence tags (ESTs) assembled into 1308 unigenes (692 contigs and 617 singletons). Quantitative analyses of the transcription levels of high redundancy clones in the ESTs revealed genes highly and predominantly expressed in laticifers, such as Rubber Elongation Factor (REF), Small Rubber Particle Protein and putative protease inhibitor proteins. HRT1 and HRT2, cis-prenyltransferases involved in rubber biosynthesis, was also expressed predominantly in laticifers, although these transcript levels were 80-fold lower than that of REF. The 5'-upstream regions of these laticifer-specific genes were cloned and analyzed in silico, revealing seven common motifs consisting of eight bases. Furthermore, transcription factors specifically expressed in laticifers were also identified. The common motifs in the laticifer-specific genes and the laticifer-specific transcription factors are potentially involved in the regulation of gene expression in laticifers. © 2014 Elsevier Ireland Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901585475&doi=10.1016%2fj.plantsci.2014.05.003&partnerID=40&md5=05de5dac0aa2dce75feb2f546ec82c62
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Transcription Factors
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Aoki Y.. "Identification of laticifer-specific genes and their promoter regions from a natural rubber producing plant Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Aoki Y.. "Identification of laticifer-specific genes and their promoter regions from a natural rubber producing plant Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Identification of laticifer-specific genes and their promoter regions from a natural rubber producing plant Hevea brasiliensis
Aoki Y.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
ETHEPON-INDUCED SPECIFIC PROTEINS ON SEVERAL RUBBER PLANT (Hevea Brasiliensis Muell. Arg) Yield Modeling and Analysing the Critical Factors of Productivity of Natural rubber (Hevea brasiliensis) Differential Expression of Methyl Jasmonate-Responsive Genes Correlates with Laticifer Vessel Proliferation in Phloem Tissue of Rubber Tree (Hevea brasiliensis) Identification of Rubber Clones (Hevea brasiliensis) Using Inter Simple Sequence Repeat (ISSR) Markers IDENTIFICATION OF DROUGHT TOLERANT GENES BY QUANTITATIVE EXPRESSION ANALYSIS IN HEVEA BRASILIENSIS Plant Hormones and Oxidative Stress in Hevea brasiliensis Effect of Temperature Constraints on Photosynthesis of Rubber (Hevea brasiliensis) ASSESSMENT OF SECONDARY LATICIFER DIFFERENTIATION IN RELATION TO RUBBER YIELD OF RUBBER TREE (HEVEA BRASILIENSIS Muell. Arg.) Identification and Molecular Characterization of Multiple forms of â-1. 3-Glucanade Gene Promoter from Hevea brasiliensis through Inverse PCR Identification of Signalling Factors involved in the Regulation of Laticifer Metabolism by Tapping and Ethephon Stimulation in Hevea brasiliensis
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