คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Kritchai Poonchareon

เผยแพร่โดย

1 Division of Biochemistry, School of Medical Sciences, University of Phayao, 19 Moo 2, Tambon Maeka, Amphur Muang, Phayao 56000. 2 Department of Medical Sciences, WHO National Salmonella and Shigella Center, National Institute of Health, Ministry of Public Health, Tiwanond Road, Amphur Muang, Nonthaburi 11000. 3 Division of Clinical Microbiology, Department of Medical Technology, School of Allied Health Sciences, University of Phayao, 19 Moo 2, Tambon Maeka, Amphur Muang, Phayao 56000.

เผยแพร่เมื่อ ไม่ระบุวันที่เผยแพร่

หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ Group B Streptococcal bacteremia burden and trends over time, 2007-15, Thailand Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand Wolbachia supergroups A and B in natural populations of medically important filth flies (Diptera : Muscidae, Calliphoridae, and Sarcophagidae) in Thailand Prevalence of zoonotic Bartonella species among rodents and shrews in Thailand Circulation of HIV-1 Multiple Complexity Recombinant Forms Among Female Sex Workers Recently Infected with HIV-1 in Thailand Draft Genome Sequence of a Clostridium botulinum Isolate from Thailand Harboring the Subtype bont/B8 Gene Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010 Mangrove forests in Thailand Progress on Rubber Breeding of Thailand Problems and needs of the elderly in Northern Thailand remote area

คัดลอก URL

ชื่อเรื่อง (EN):	Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among Salmonella enterica Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN):	Kritchai Poonchareon
บทคัดย่อ (EN):	Salmonella enterica Serotype 4,[5],12:i:-, a monophasic variant of S. Typhimurium, with high virulence and multidrug resistance is distributed globally causing pathogenicity to both humans and domesticated animals. BOX-A1R-based repetitive extragenic palindromic-PCR (BOX)-PCR proved to be superior to three other repetitive element-based PCR typing methods, namely, enterobacterial repetitive intergenic consensus (ERIC)-, poly-trinucleotide (GTG)5-, and repetitive extragenic palindromic (REP)-PCR (carried out under a single optimized amplification condition), in differentiating genetic relatedness among S. 4,[5],12:i:-isolates from feces of hospitalized patients (n = 12) and isolates from minced pork samples of S. 4,[5],12:i:- (n = 6), S. Typhimurium (n = 6), and Salmonella Serogroup B (n = 4) collected from different regions of northern ,Thailand. Construction of phylogenetic trees from amplicon size patterns allowed allocation of Salmonella isolates into clusters of similar genetic relatedness, with BOX-PCR generating more unique clusters for each serotype than the other three typing methods. BOX-, (GTG)5-, and REPPCR indicated significant genetic relatedness between S. 4,[5],12:i:- isolates 1 and 9 from hospitalized patients and S. 4,[5],12:i:- isolate en 29 from minced pork, suggesting a possible route of transmission. ,us, BOX-PCR provides a suitable molecular typing method for discriminating genetic relatedness among Salmonella spp. of the same and different serotypes and should be suitable for application in typing and tracking route of transmission in Salmonella outbreaks.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=551
เผยแพร่โดย (EN):	1 Division of Biochemistry, School of Medical Sciences, University of Phayao, 19 Moo 2, Tambon Maeka, Amphur Muang, Phayao 56000. 2 Department of Medical Sciences, WHO National Salmonella and Shigella Center, National Institute of Health, Ministry of Public Health, Tiwanond Road, Amphur Muang, Nonthaburi 11000. 3 Division of Clinical Microbiology, Department of Medical Technology, School of Allied Health Sciences, University of Phayao, 19 Moo 2, Tambon Maeka, Amphur Muang, Phayao 56000.
คำสำคัญ (EN):	Effectiveness of BOX-PCR
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science