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Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis)
Thakurdas Saha - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis)
ผู้แต่ง / หัวหน้าโครงการ (EN): Thakurdas Saha
บทคัดย่อ (EN): Increase in lignification is often observed in response to pathogen attack. Since lignin is a non-degradable mechanical barrier for most microorganisms, it increased the tolerance of the host by blocking pathogen invasion. Plant O-methyltransferases (OMTs) play important roles in secondary metabolism through pheny1 propanoid pathway. The full-length HCOMT cDNA (1347 bp in size) encoding caffeic acid O-methyltransferase consisting of 369 amino acids was cloned and characterized from the bark tissue of a cultivated rubber Clone RRII 118. Southern analysis of the genomic DNA using a partial fragment of HCOMT as probe revealed the presence of multiple forms of the gene. The involvement of HCOMT in disease tolerance was assessed through real time quantification of gene expression after challenging tolerant (GT 1) and susceptible (RRII 105) clones of rubber with Corynespora cassiicola. Spore suspension was sprayed on young emerging leaves of RRII 105 and GT 1 and covered with a polybag to maintain mumidity essential for establishment of the pathogen on the host. Control plants were also maintained in a similar condition by spraying sterile water on to the leaves. RNA was isolated from the leaves collected at different time intervals (0, 4, 12 and 24 h) following inoculation and converted to cDNA for real time quantification of the expression using specific primer-pairs designed from the 3’ end of the gene including partial coding sequence and 3’UTR. Differential expression of the gene HCOMT was noticed between these two clones, even in unchallenged condition and expressin level was more in RRII 105 over the tolerant clone GT1. However, at 4 hours following infection, the expression levels shot up in both the cased. At 12 hours, expression level was found to reduce sighificantly (12 fold compared to the expression level at 4 hours) only in case of RRII 105. Even though anincreasing trend of gene expression level was noticed in RRII 105 at 24 h of infection, it couldn’t reach up to the control level. Whereas in GT1, expression shot up significantly after 12 h and eventually at 24 h it had increased by 62 fold compared to the control indication that this form of OMT might participate in the hypersensitive reaction in GT1 in response to Corynespora cassiicola infection. From this study it appears that gradual increment in expression of HCOMT gene in GT1 after the infection in initial hours followed by a sudden boost during the period from 12 to 24 h when the pathogen establishes on the host could be one of the reasons for the tolerance observed in GT1. Further work is in progress to identify other forms of OMTs and their functional role in disease tolerance in rubber.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): http://clinic.rubberboard.org.in/PDF/ircpdf/BT-08%20Saha%20Presentation_IRC-for%20web.pdf
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): Corynespora Leaf Disease
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Thakurdas Saha. "Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis)". ไม่ระบุหน่วยงาน. ม.ป.ป.

Thakurdas Saha. "Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis)". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis)
Thakurdas Saha
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platf Identification of Resistance of Hevea brasiliensis Clones to Corynespora Leaf Fall Disease Rapid Molecular Identification and Detection of Corynespora cassiicola of Hevea brasiliensis Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis) Surveillance on Corynespora Leaf Fall Disease: Incidence and Severity on Natural Rubber (Hevea brasiliensis) in Certain Hot Spots Areas in Kerala THE YIELD AND QUALITY OF RUBBER (HEVEA BRASILIENSIS) AS AFFECTED BY IRRIGATION AND FERTILIZER APPLICATIONS CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN Yield Modeling and Analysing the Critical Factors of Productivity of Natural rubber (Hevea brasiliensis) Lipid composition of Hevea brasiliensis latex and dry rubber: characterization and relation with some physical properties Functional Characterization of a Novel Hevea â-1, 3-Glucanase Gene Promoter and Its Regulatory Role in Hevea and Tobacco Tissues
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