สืบค้นงานวิจัย
Paper-based acetylcholinesterase inhibition assay combining a wet system for organophosphate and carbamate pesticides detection
Apilux A. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Paper-based acetylcholinesterase inhibition assay combining a wet system for organophosphate and carbamate pesticides detection
ผู้แต่ง / หัวหน้าโครงการ (EN): Apilux A.
บทคัดย่อ (EN): A dramatic increase in pesticide usage in agriculture highlights the need for on-site monitoring for public health and safety. Here, a paper-based sensor combined with a wet system was developed for the simple and rapid screening of organophosphate (OP) and carbamate (CM) pesticides based on the inhibition of acetylcholinesterase (AChE). The paper-based sensor was designed as a foldable device consisting of a cover and detection sheets pre-prepared with indoxyl acetate and AChE, respectively. The paper-based sensor requires only the incubation of a sample on the test zone for 10 minutes, followed by closing of the foldable sheet to initiate the enzymatic reaction. Importantly, the buffer loading hole was additionally designed on the cover sheet to facilitate the interaction of the coated substrate and the immobilized enzyme. This subsequently facilitates the mixing of indoxyl acetate with AChE, resulting in the improved analytical performance of the sensor. The absence or decrease in blue color produced by the AChE hydrolysis of indoxyl acetate can be observed in the presence of OPs and CMs. Under optimized conditions and using image analysis, the limit of detection (LOD) of carbofuran, dichlorvos, carbaryl, paraoxon, and pirimicarb are 0.003, 0.3, 0.5, 0.6, and 0.6 ppm, respectively. The assay could be applied to determine OP and CM residues in spiked food samples. Visual interpretation of the color signal was clearly observed at the concentration of 5 mg/kg. Furthermore, a self-contained sample pre-concentration approach greatly enhanced the detection sensitivity. The paper-based device developed here is low-cost, requires minimal reagents and is easy to handle. As such, it would be practically useful for pesticide screening by nonprofessional end-users. © 2015, Leibniz Research Centre for Working Environment and Human Factors. All rights reserved.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84923651337&partnerID=40&md5=fb101549db6d8c4d9aec2aa0eeb0be34
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): wet system
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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Apilux A.. "Paper-based acetylcholinesterase inhibition assay combining a wet system for organophosphate and carbamate pesticides detection". ไม่ระบุหน่วยงาน. ม.ป.ป.

Apilux A.. "Paper-based acetylcholinesterase inhibition assay combining a wet system for organophosphate and carbamate pesticides detection". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Paper-based acetylcholinesterase inhibition assay combining a wet system for organophosphate and carbamate pesticides detection
Apilux A.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Pesticide analysis using nanoceria-coated paper-based devices as a detection platform Development of molecular approach based on PCR assay for detection of histamine producing bacteria COMMUNITY-BASED PARTICIPATORY ACTION RESEARCH (CPAR) ON RUBBER-BASED FARMING SYSTEM IN THE PHILIPPINES Rubber-Based Farming System in Southern Philippines A silicon nitride ISFET based immunosensor for Ag85B detection of tuberculosis Investigation on a new rubber based farming system in China Immunodiagnosis of fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and Immunochromatographic Assay for detection of circulating cathepsin L1 Protease Development of botulinum toxin detection assay by antibody capture ELISA RUBBER-BASED CROPPING SYSTEM IN NIGERIA: EFFORTS IN RESEARCH AND EXTENSION JDM88 AGRO-VENTURES RUBBER BASED FARMING SYSTEM
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