สืบค้นงานวิจัย
Three novel mutations in α-galactosidase gene involving in galactomannan degradation in endosperm of curd coconut
Phoeurk C. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Three novel mutations in α-galactosidase gene involving in galactomannan degradation in endosperm of curd coconut
ผู้แต่ง / หัวหน้าโครงการ (EN): Phoeurk C.
บทคัดย่อ (EN): The deficiency of α-galactosidase activity in coconut endosperm has been reported to cause a disability to hydrolyze oligogalactomannan in endosperm resulting in curd coconut phenotype. However, neither the α-galactosidase encoding gene in coconut nor the mutation type has been identified and characterized in normal and curd coconuts. In this study, cDNA and genomic DNA encoding α-galactosidase gene alleles from a normal and two curd coconuts were successfully cloned and characterized. The deduced amino acid of wild type α-galactosidase contains 398 amino acid residues with a 17 N-terminal amino acids signal peptide sequence. Three mutant alleles, the first 19-amino acids from 67 to 85 (ADALVSTGLARLGYQYVNL) deletion with S137R and the second R216T, were identified from curd coconut plant no.1 while the third P250R was identified from curd coconut plant no. 10. All mutations of α-galactosidase gene were confirmed by the analysis of parental genomic DNA from normal and curd coconuts. Heterologous expression in Komagataella phaffii (Pichia pastoris) indicated that recombinant P250R, R216T and 19-amino acids deletion-S137R mutant proteins showed no α-galactosidase activity. Only the recombinant wild-type protein was able to detect for α-galactosidase activity. These results are in accordance with the no detection of α-galactosidase activity in developing curd coconut endosperms by tissue staining. While, the accumulation of enzyme activity was present in the solid endosperm of normal coconut. The full-length cDNA and parental genomic DNA sequences encoding α-galactosidase in normal coconut as well as identified curd coconut mutant alleles are reported in Genbank accession no. KJ957156 and KM001681-3. Transcription level of the α-galactosidase gene in mature curd coconut endosperm was at least 20 times higher than normal. In conclusion, absence of α-galactosidase activity caused by gene mutations associates with an accumulation of oligogalactomannan in endosperms, resulting in curd coconut phenotype. © 2018 Elsevier Ltd
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052437931&doi=10.1016%2fj.phytochem.2018.08.015&partnerID=40&md5=ebc930b7765c0086505ccce9593d3e4c
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Sequence Alignment
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Phoeurk C.. "Three novel mutations in α-galactosidase gene involving in galactomannan degradation in endosperm of curd coconut". ไม่ระบุหน่วยงาน. ม.ป.ป.

Phoeurk C.. "Three novel mutations in α-galactosidase gene involving in galactomannan degradation in endosperm of curd coconut". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Three novel mutations in α-galactosidase gene involving in galactomannan degradation in endosperm of curd coconut
Phoeurk C.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Analysis of Novel Mutations and Methylmalonyl-CoA Mutase Levels in Thai Patients with Isolated Methylmalonic Acidemia (ภาษาอังกฤษ) Functional characterization of a novel C-type lectin gene in the black tiger shrimp, Penaeus monodon New higher taxa in the lichen family Graphidaceae (lichenized Ascomycota : Ostropales) based on a three-gene skeleton phylogeny A novel SNP (c.258+43C GT T) in LPL gene and association with milk production in buffaloes (Buffalo Bulletin,38(2)) Functional Characterization of a Novel Hevea â-1, 3-Glucanase Gene Promoter and Its Regulatory Role in Hevea and Tobacco Tissues Identification of putative kdr mutations in the tropical bed bug, Cimex hemipterus (Hemiptera : Cimicidae) Eggplant fruits protect against DNA damage and mutations Cloning of Arabidopsis WUS Gene, Construcion and Identification of WUS-EGFP Fusion Gene Plant Expression Vector Naturally Occurring Mutations in HIV-1 CRF01_AE Capsid Affect Viral Sensitivity to Restriction Factors Effect of Insulin like growth factor 1 gene, Insulin like growth factor 2 gene and Melanocortin 4 receptor gene on growth performance and carcass traits in Thai indigenous chicken (Leung Hang Khao)
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair