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Identification of veillonella species in the tongue biofilm by using a novel one-step polymerase chain reaction method
Mashima I. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Identification of veillonella species in the tongue biofilm by using a novel one-step polymerase chain reaction method
ผู้แต่ง / หัวหน้าโครงการ (EN): Mashima I.
บทคัดย่อ (EN): Six Veillonella species have been frequently isolated from human oral cavities including infectious sites. Recently, it was reported that diet, smoking, and possibly socioeconomic status can influence the bacterial profile in oral cavities. In addition, oral hygiene habits may also influence oral microbiota in terms of both numbers and diversity of microorganisms. In this study, the identification of Veillonella species in tongue biofilms of Thai children, divided into three groups dependent on their status of oral hygiene. For this, we used a novel onestep PCR method with species-specific primer sets based on sequences of the rpoB gene. As shown in the results, the number of isolates of Veillonella species was 101 strains from only 10 of 89 subjects. However, the total number of bacteria was high for all subjects. Since it was reported in previous studies that Veillonella species were easy to isolate in human tongue biofilms at high numbers, the results obtained in this study may suggest country- or age-specific differences. Moreover, Veillonella species were detected predominantly in subjects who had poor oral hygiene compared to those with good or moderate oral hygiene. From these results, there is a possibility that Veillonella species may be an index of oral hygiene status. Furthermore, V. rogosae was a predominant species in tongue biofilms of Thai children, whereas V. parvula and V. denticariosi were not isolated at all. These characteristics of the distribution and frequency of Veillonella species are similar to those reported in previous studies. Although further studies are needed in other countries, in this study, a successful novel one-step PCR method was established to detect Veillonella species in human oral cavities easily and effectively. Furthermore, this is the first report investigating the distribution and frequency of Veillonella species in tongue biofilms of Thai children. © 2016 Mashima et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84976907893&doi=10.1371%2fjournal.pone.0157516&partnerID=40&md5=6f11a9913226d196d440f69e4628db50
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Veillonella
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Identification of veillonella species in the tongue biofilm by using a novel one-step polymerase chain reaction method
Mashima I.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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