สืบค้นงานวิจัย
Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol
Ara F. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol
ผู้แต่ง / หัวหน้าโครงการ (EN): Ara F.
บทคัดย่อ (EN): Vitellogenin gene (Vg) expression was investigated in the giant freshwater prawn, Macrobrachium rosenbergii at different ovarian stages and after exposure to 4-nonylphenol (4-NP), a widespread xeno-oestrogen present in aquatic environments. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed a high level of Vg expression in the hepatopancreas at all ovarian stages, but at a much lower level in the ovary and real-time PCR showed differential Vg expression in both the hepatopancreas and ovary. Vg expression in the hepatopancreas increased significantly at the mature ovarian stage. The expression profile in the ovary was the inverse of that in the hepatopancreas. Furthermore, the hepatopancreas was more responsive to the oestrogenic activity of 4-NP. Exposure to 50-250 μg L-1 4-NP for 96 h increased Vg expression in the hepatopancreas in a dose-dependent manner, whereas Vg expression was less in the ovary. In conclusion, Vg expression was tissue- and stage-dependent. These factors must be considered when Vg expression is used as an indicator of reproductive activity in an organism or for assessing xeno-oestrogens in the environments. © 2012 John Wiley & Sons Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84892436325&doi=10.1111%2fj.1365-2109.2012.03229.x&partnerID=40&md5=a9cd3e7da0cb6d36e1147347d732aabd
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Macrobrachium rosenbergii
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol
Ara F.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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