สืบค้นงานวิจัย
Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platform
C. Bindu Roy - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platform
ผู้แต่ง / หัวหน้าโครงการ (EN): C. Bindu Roy
บทคัดย่อ (EN): Corynespora leaf disease caused by Corynespora cassiicola is one of the major diseases causing considerable reduction in the yield of natural rubber. Next Generation Sequencing (NGS) based RNA-Seq technology (transcriptome sequencing) and de novo transcriptome assembly was adopted to gain a comprehensive overview of the Hevea brasiliensis transcriptome in response to pathogen infection. Two clones of rubber: RRII 105 (Corynespora susceptible) and GT 1 (Corynespora—tolerant) were used in the study to understand the genes involved in host tolerance during Corynespora leaf disease development in rubber. Transcriptomes of the control (unchallenged) and treated (Corynespora challenged) samples obtained from Illumina HiSeq 2000 were compared. The sequencing output generated more than 134 million high quality reads in control and treated samples of both the clones, which were assembled using Velvet 1.2.07 fikkiwed bt Iases 0.1.21 with optimal parameters into an average of 124137 contigs and 105405 transcripts with an average length of 440 and 1373 bases for contigs and transcripts respectively. The average N50 value for the contigs and transcripts were 665 and 2097 bases respectively. A total of 13239 and 19084 simple sequence repeats were detected in the control and treated samples, some being common and others being unique to both the clones and treatments. All types of repeat units (mono, di, tri, tetra, penta and hexa nucleotide) were observed with the majority being mononucleotide repeats (48%), followed by di mucleotide repeats (18.3%). The transcripts were annotated through homology search with Ricinus communis, Populus spp. and Arabidopsis thaliana against the genomic resources available at the GenBank. A comparison of the differential gene expression data revealed that an average of 397 and 649 genes were down regulated and upregulated respectively when comparing the control transcriptomes of the two clones. Upon challenge inoculation, it was observed that 58 genes were down regulated and 574 genes were up regulated in the clone RRII 105 when subjected to pathogeninfection. Whereas, in the case of the tolerant clone GT1, 5 genes were down regulated and 817 genes were upregulated following pathogen infection. Furthermore, the annotated genes were functionally classified according to the gene ontology. The data from this study provides a comprehensive transcript resource for understanding the response of rubber trees to the pathogen C. cassiicola. Which is also a resource for gene discovery and development of functional molecular markets. This study further demonstrates effective use of Illumina HiSeq 2000 sequencing data for de novo transcriptome assembly for the commercially important crop Hevea brasilliensis.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): Corynespora cassiicola
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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C. Bindu Roy. "Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platform". ไม่ระบุหน่วยงาน. ม.ป.ป.

C. Bindu Roy. "Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platform". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platform
C. Bindu Roy
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Structural and Functional Characterization of a NBS-LRR Disease Resistance Gene Associated With Tolerance against Corynespora Cassiicola Causing Leaf Fall Disease in Rubber (Hevea brasiliensis) Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis) Identification of Resistance of Hevea brasiliensis Clones to Corynespora Leaf Fall Disease Effect of Temperature Constraints on Photosynthesis of Rubber (Hevea brasiliensis) Surveillance on Corynespora Leaf Fall Disease: Incidence and Severity on Natural Rubber (Hevea brasiliensis) in Certain Hot Spots Areas in Kerala Rapid Molecular Identification and Detection of Corynespora cassiicola of Hevea brasiliensis Identification of Rubber Clones (Hevea brasiliensis) Using Inter Simple Sequence Repeat (ISSR) Markers CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN CORYNESPORA LEAF FALL: SUCCESS STORY OF THE MANAGEMENT IN SRILANKA AND FUTURE OF THE WORLD’S LEADING RUBBER CLONES GENETIC MAPPING AND IDENTIFICATION OF QTLS INVOLVED IN THE TOLERANCE OF RUBBER TREE (HEVEA BRASILIENSIS) TO CORYNESPORA CASSIICOLA SECRETED EFFECTRORS
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