สืบค้นงานวิจัย
Functional characterization of recombinant gonad-inhibiting hormone (GIH) and implication of antibody neutralization on induction of ovarian maturation in marine shrimp
Treerattrakool S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Functional characterization of recombinant gonad-inhibiting hormone (GIH) and implication of antibody neutralization on induction of ovarian maturation in marine shrimp
ผู้แต่ง / หัวหน้าโครงการ (EN): Treerattrakool S.
บทคัดย่อ (EN): Ovarian maturation in crustacean is controlled by an eyestalk neuropeptide called gonad-inhibiting hormone (GIH) that is presumed to inhibit vitellogenin synthesis. In this study, a recombinant protein of GIH of Penaeus monodon (rPem-GIH) was expressed in Pichia pastoris expression system and purified to homogeneity by reversed-phase SPE (C18). The purified rPem-GIH significantly reduced vitellogenin mRNA level in primary tissue culture derived from previtellogenic ovary of P. monodon broodstock by 45.7% compared with the untreated group. This effect was similar to that of partially purified optic lobe extract, and demonstrated that the rPem-GIH possessed gonad-inhibiting activity. A monoclonal antibody specific to Pem-GIH (anti-GIH mAb) was able to neutralize the activity of GIH from the partially purified optic lobe extract to about 58% by in vitro assay in primary tissue culture of the ovary. Peptide mapping revealed that amino acids 16 to 20 (MYNKV) of the mature Pem-GIH may serve as an epitope of anti-GIH mAb. A single injection of anti-GIH mAb into wild previtellogenic P. monodon was able to induce ovarian maturation and spawning at a comparable rate to the eyestalk ablation. Our results demonstrate neutralization effect of anti-GIH mAb on vitellogenesis inhibitory action of GIH, and thus provide a potential alternative to induce ovarian maturation in P. monodon broodstock. © 2014 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897393061&doi=10.1016%2fj.aquaculture.2014.03.009&partnerID=40&md5=ab9a0e6d3d447f4184afccdef0c05d9b
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): yeast
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Treerattrakool S.. "Functional characterization of recombinant gonad-inhibiting hormone (GIH) and implication of antibody neutralization on induction of ovarian maturation in marine shrimp". ไม่ระบุหน่วยงาน. ม.ป.ป.

Treerattrakool S.. "Functional characterization of recombinant gonad-inhibiting hormone (GIH) and implication of antibody neutralization on induction of ovarian maturation in marine shrimp". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Functional characterization of recombinant gonad-inhibiting hormone (GIH) and implication of antibody neutralization on induction of ovarian maturation in marine shrimp
Treerattrakool S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Correlation between gonad-inhibiting hormone and vitellogenin during ovarian maturation in the domesticated Penaeus monodon Effects of gonadotropin-releasing hormones and dopamine on ovarian maturation in the Pacific white shrimp, Litopenaeus vannamei, and their presence in the ovary during ovarian development The presence of abalone egg-laying hormone-like peptide in the central nervous system and ovary of the blue swimming crab, Portunus pelagicus, and its effect on ovarian maturation Characterization of an abalone gonadotropin-releasing hormone and its effect on ovarian cell proliferation A novel localization of molt-inhibiting hormone in the tegumental glands of shrimp Penaeus monodon and its possible role in shrimp molting Identification and characterization of a crustacean female sex hormone in the giant freshwater prawn, Macrobrachium rosenbergii In vitro study of a putative role of gonad-inhibiting hormone in oocyte growth stimulation in Penaeus monodon In vitro neutralization of yellow head virus infection in shrimp using recombinant PmYRP65 protein Neuropeptide F stimulates ovarian development and spawning in the female giant freshwater prawn, Macrobrachium rosenbergii, and its expression in the ovary during ovarian maturation cycle Production and characterization of a monoclonal antibody specific to 16 kDa antigen of Paramphistomum gracile
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