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Effect of antioxidant additives on freezability of buffalo spermatozoa (Buffalo Bulletin,39(3))
Ranjana Sinha - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Effect of antioxidant additives on freezability of buffalo spermatozoa (Buffalo Bulletin,39(3))
ผู้แต่ง / หัวหน้าโครงการ (EN): Ranjana Sinha
หน่วยงานสังกัดผู้แต่ง (EN): Haryana (India). Indian Council of Agricultural Research. Artificial Breeding Research Center
บทคัดย่อ (EN): The cryopreservation induced sperm damage in buffalo is one of the hurdles responsible for its poor freezability and fertility. Therefore, the Present study was planned to understand the role of antioxidant additives on freezability of buffalo spermatozoa. Twenty four ejaculates were collected having mass motility GT= +3 from 4 Murrah bulls (6 from each bull). Each ejaculate was divided into four groups viz., Group 1 as Control containing Tris-egg yolk-glycerol extender, Group 2 containing Pentoxiphylline 3.6 mM, Group 3 containing Theophylline 10 mM, Group 4 containing Theobromine 10 mM, Group 5 containing N-propyl gallate 15 micro M and finally diluted upto 80*10**(6) sperm/ml. Ejaculates were evaluated after freezing for seminal attribute viz., individual motility, live sperm, acrosomal integrity (AI) and hypo-osmotic swelling test (HOST). One way ANOVA was used to analyse the data. Significantly (P LT 0.05) higher sperm motility was observed in semen samples treated with pentoxiphylline in comparison to control, theophylline and theobromine and propyl gallate treated semen. At day 0, 7 and 30 days, semen treated with pentoxiphylline had significantly (P LT 0.05) higher percentage of viable spermatozoa as compared to theophylline and theobromine and propyl gallate treated semen. HOST and AI was significantly (P LT 0.05) higher in theophylline, theobromine and propyl gallate treated semen, however, among additive treated groups, HOST and AI were significantly (P LT 0.05) higher in pentoxiphylline treated semen. In conclusion among various antioxidant additives, pentoxyphylline addition 3.6 mM significantly improved semen quality of buffalo bulls.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/402540
คำสำคัญ (EN): India
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

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