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Double Dengue Serotypes in Asymptomatic Populations Living in An Area of Thailand Endemic for Dengue Hemorrhagic Fever
Veerayuth Kittichai - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Double Dengue Serotypes in Asymptomatic Populations Living in An Area of Thailand Endemic for Dengue Hemorrhagic Fever
ผู้แต่ง / หัวหน้าโครงการ (EN): Veerayuth Kittichai
บทคัดย่อ (EN): Dengue virus infection remains a major health problem worldwide. Understanding dengue infection and characterizing circulating viruses are essential for disease prevention and control as well as vaccine development. In this study, we aimed to identify dengue virus in healthy people living in an area endemic for dengue disease. Blood samples were collected from 52 healthy local subjects living in a dengue-endemic area of Thailand. Viral RNA was detected using a nested reverse transcription polymerase chain (RT-PCR) that amplified the E gene. Phylogenetic trees were constructed by the neighbor-joining method using MEGA6.06. Dengue virus was detected in 5 of 52 samples (9.62%). Double dengue virus infection (Den 1&4 and Den 3&4) was found in two samples. Sequence analysis of the viruses showed that dengue serotype 1 belonged to sylvatic genotype. A mix of genotype I and II was found in a dengue serotype 3 sample while all dengue virus serotype 4 in this study belonged to genotype II. These preliminary results may provide better understanding of dengue infection and viral transmission between populations and mosquitoes. Therefore, it could be used for epidemiological studies and control of dengue hemorrhagic fever in the future.
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ภาษา (EN): en
เอกสารแนบ (EN): http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=472
เผยแพร่โดย (EN): 1 Medical Science Program, Faculty of Medicine, Chulalongkorn University, Bangkok 10300, Thailand 2 Department of Clinical Microbiology, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540, Thailand 3 Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan 10540, Thailand 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand 5 Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand 6 Excellence Center for Emerging Infectious Disease, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand
คำสำคัญ (EN): phylogenetic tree
เจ้าของลิขสิทธิ์ (EN): National Institute of Health, Department of Medical Science
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Double Dengue Serotypes in Asymptomatic Populations Living in An Area of Thailand Endemic for Dengue Hemorrhagic Fever
Veerayuth Kittichai
1 Medical Science Program, Faculty of Medicine, Chulalongkorn University, Bangkok 10300, Thailand 2 Department of Clinical Microbiology, Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, 10540, Thailand 3 Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan 10540, Thailand 4 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand 5 Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand 6 Excellence Center for Emerging Infectious Disease, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand
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