คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Wichuda Phothichaisri

เผยแพร่โดย

1 Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand. 2 Department of Biology, Faculty ofScience, Mahidol University, Bangkok, Thailand. 3 Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand. 4 Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi, Thailand.

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งานวิจัยที่แนะนำ Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010 Antimicrobial susceptibility and toxin production of Clostridium difficile isolated from diarrheal patients during 2012-2015 Molecular epidemiology of Clostridium difficile infection in a large teaching hospital in Thailand ผลงานนำเสนอประชุมวิชาการกระทรวงสาธารณสุข ปีงบประมาณ 2559 เรื่อง ความไวของเชื้อ Clostridium difficile ต่อยาต้านจุลชีพด้วยวิธี agar dilution (2555 - 2558) Clostridium botulinum Occurrence of bacteriophages infecting Aeromonas, Enterobacter, and Klebsiella inwater and association with contamination sources in Thailand Characterization and genetic variation of vibrio cholerae isolated from clinical and environmental sources in Thailand การปนเปื้อนของแบคทีเรีย Clostridium botulinum ในผลิตภัณฑ์นมจากประเทศนิวซีแลนด์ Prevalence of toxigenic Clostridium perfringens strains isolated from dried spur pepper in Thailand การศึกษาคุณลักษณะทางแบคทีเรียและทางพันธุกรรมของ Morganella morganii ที่แยกได้จากตัวอย่างอุจจาระของคนในประเทศไทย Bacteriological and genomic characterization of clinical isolates of Morganella morganii

คัดลอก URL

ชื่อเรื่อง (EN):	Characterization of Bacteriophages Infecting Clinical Isolates of Clostridium difficile
ผู้แต่ง / หัวหน้าโครงการ (EN):	Wichuda Phothichaisri
บทคัดย่อ (EN):	Clostridium difficile is recognized as a problematic pathogen, causing severe enteric diseases including antibiotic-associated diarrhea and pseudomembranous colitis. The emergence of antibiotic resistant C. difficile has driven a search for alternative anti-infection modalities. A promising strategy for controlling bacterial infection includes the use of bacteriophages and their gene products. Currently, knowledge of phages active against C. difficile is still relatively limited by the fact that the isolation of phages for this organism is a technically demanding method since bacterial host themselves are difficult to culture. To isolate and characterize phages specific to C. difficile, a genotoxic agent, mitomycin C, was used to induce temperate phages from 12 clinical isolates of C. difficile. Five temperate phages consisting of 8HR24, 8HN10, 8HN16-1, 8HN16-2, and 8HN50 were successfully induced and isolated. Spotting assays were performed against a panel of 92 C. difficile isolates to screen for susceptible bacterial hosts. The results revealed that all the C. difficile phages obtained in this work displayed a relatively narrow host range of 0–6.5% of the tested isolates. Electron microscopic characterization revealed that all isolated phages contained an icosahedral head connected to a long contractile tail, suggesting that they belonged to the Myoviridae family. Restriction enzyme analysis indicated that these phages possess unique double-stranded DNA genome. Further electron microscopic characterization revealed that the 8HN10 absorbed to the bacterial surface via attachment to cell wall, potentially interacting with S-layer protein. Bacteriophages isolated from this study could lead to development of novel therapeutic agents and detection strategies for C. difficile.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=530
เผยแพร่โดย (EN):	1 Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand. 2 Department of Biology, Faculty ofScience, Mahidol University, Bangkok, Thailand. 3 Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand. 4 Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi, Thailand.
คำสำคัญ (EN):	Clostridium difficile; temperate phage; phage induction; host range; Myoviridae
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science