สืบค้นงานวิจัย
Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
Turathum B. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
ผู้แต่ง / หัวหน้าโครงการ (EN): Turathum B.
บทคัดย่อ (EN): The domestic cat serves as an animal model for assisted reproductive studies of endangered felid species. To date, there are no data on the protein alterations following cryopreservation of oocytes in felid family. Methods: Immature (germinal vesicle) domestic cat oocytes were vitrified in the vitrification solution containing 35% ethylene glycol, 20% DMSO and 0.5 mM sucrose. The vitrified-warmed oocytes were matured (metaphase II) in vitro and subjected to proteomic analysis using 1DE SDS-PAGE prefractionation combined with LC-MS/MS. Results: A total of 1712 proteins were identified in in vitro matured oocytes. Of the 1712 proteins, 1454 proteins were found in both groups, whereas, 258 proteins were differentially expressed between control and vitrified-warmed groups. In vitrified-warmed oocytes, the missing proteins were membrane and nuclear proteins; whereas, apoptosis and DNA repair proteins were overrepresented. Conclusions: The identified missing and overexpressed proteins in vitrified-warmed oocytes represent potential markers of cryoinjuries and the developmental pathways of oocytes. The findings of differential expressed proteins may contribute to effective ways of proteome analysis of oocyte/embryo quality in order to assess safety of cryopreservation in felid species. © The Author(s) 2017.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053732447&doi=10.1186%2fs40659-018-0176-5&partnerID=40&md5=4723f4eba07a8072e6d8b9f002ce8bf6
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Vitrification
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Turathum B.. "Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Turathum B.. "Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
Turathum B.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Correlation between gonad-inhibiting hormone and vitellogenin during ovarian maturation in the domesticated Penaeus monodon Proteomic analysis of the schistosoma mansoni miracidium Proteomic analysis and white spot syndrome virus interaction of mud crab (Scylla olivacea) revealed responsive roles of the hemocytes In vitro study of a putative role of gonad-inhibiting hormone in oocyte growth stimulation in Penaeus monodon Insights of Hevea Latex Serum Proteins Using Shotgun Proteomics Analysis Comparative proteomic analysis of Chlamydomonas reinhardtii control and a salinity-tolerant strain revealed a differential protein expression pattern Metal complexation by histidine-rich peptides confers protective roles against cadmium stress in Escherichia coli as revealed by proteomics analysis Changes of fatty acids in phosphatidylcholine on sperm membrane during Macrobrachium rosenbergii sperm transit through spermatic duct and lipid analysis in spermatic vesicles Effect of extracellular adenosine 5'-triphosphate on cryopreserved epididymal cat sperm intracellular ATP concentration, sperm quality, and in vitro fertilizing ability การรอดชีวิตของตัวอ่อนแพะที่แช่แข็งโดยวิธี Conventional Slow Freezing และ Vitrification
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