สืบค้นงานวิจัย
Molecular cloning and characterization of Fasciola gigantica thioredoxin-glutathione reductase
Changklungmoa N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Molecular cloning and characterization of Fasciola gigantica thioredoxin-glutathione reductase
ผู้แต่ง / หัวหน้าโครงการ (EN): Changklungmoa N.
บทคัดย่อ (EN): The Fasciola gigantica thioredoxin-glutathione reductase (FgTGR) gene is a fusion between thioredoxin reductase (TR) and a glutaredoxin (Grx) gene. FgTGR was cloned by polymerase chain reaction (PCR) from adult complementary DNA (cDNA), and its sequences showed two isoforms, i.e., the cytosolic and mitochondrial FgTGR. Cytosolic FgTGR (cytFgTGR) was composed of 2370 bp, and its peptide had no signal sequence and hence was not a secreted protein. Mitochondrial FgTGR (mitFgTGR) was composed of 2506 bp with a signal peptide of 43 amino acids; therefore, it was a secreted protein. The putative cytFgTGR and mitFgTGR peptides comprised of 598 and 641 amino acids, respectively, with a molecular weight of 65.8 kDa for cytFgTGR and mitFgTGR, with a conserved sequence (CPYC) of TR, and ACUG and CVNVGC of Grx domains. The recombinant FgTGR (rFgTGR) was expressed in Escherichia coli BL21 (DE3) and used for production for a polyclonal antibody in rabbits (anti-rFgTGR). The FgTGR protein expression, estimated by indirect ELISA using the rabbit anti-rFgTGR as probe, showed high levels of expression in eggs, and 2- and 4-week-old juveniles and adults. The rFgTGR exhibited specific activities in the 5,5′-dithiobis (2-nitro-benzoic acid) (DTNB) reductase assay for TR activity and in β-hydroxyethul disulfide (HED) for Grx activity. When analyzed by immunoblotting and immunohistochemistry, rabbit anti-rFgTGR reacted with natural FgTGR at a molecular weight of 66 kDa from eggs, whole body fraction (WB) of metacercariae, NEJ, 2- and 4-week-old juveniles and adults, and the tegumental antigen (TA) of adult. The FgTGR protein was expressed at high levels in the tegument of 2- and 4-week-old juveniles. The FgTGR may be one of the major factors acting against oxidative stresses that can damage the parasite; hence, it could be considered as a novel vaccine or a drug target. © 2015, Springer-Verlag Berlin Heidelberg.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939997449&doi=10.1007%2fs00436-015-4400-8&partnerID=40&md5=3a72dce3959510a37971720cb54d6fbb
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Thioredoxins
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Changklungmoa N.. "Molecular cloning and characterization of Fasciola gigantica thioredoxin-glutathione reductase". ไม่ระบุหน่วยงาน. ม.ป.ป.

Changklungmoa N.. "Molecular cloning and characterization of Fasciola gigantica thioredoxin-glutathione reductase". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Molecular cloning and characterization of Fasciola gigantica thioredoxin-glutathione reductase
Changklungmoa N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Expression and characterization of glutathione peroxidase of the liver fluke, Fasciola gigantica Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii Monoclonal antibody against recombinant Fasciola gigantica cathepsin L1H could detect juvenile and adult cathepsin Ls of Fasciola gigantica Cloning and Characterization of Specific Molecular Markers of Rubber Tree Trunk Phloem Necrosis Molecular cloning and characterization of Mj-mov-10, a putative RNA helicase involved in RNAi of kuruma shrimp Molecular detection and characterization of noroviruses in river water in Thailand The in vitro anthelmintic activity of the ethanol leaf extracts of Terminalia catappa L. On Fasciola gigantica Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii Cloning and expression of 3-hydroxy-3-methylglutaryl-Coa Reductase 1 (Hmge 1) from Hevea brasiliensis Molecular characterization of serine protease inhibitor isoform 3, SmSPI, from Schistosoma mansoni
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair