สืบค้นงานวิจัย
Development of molecular approach based on PCR assay for detection of histamine producing bacteria
Wongsariya K. - มหาวิทยาลัยมหิดล
ชื่อเรื่อง (EN): Development of molecular approach based on PCR assay for detection of histamine producing bacteria
ผู้แต่ง / หัวหน้าโครงการ (EN): Wongsariya K.
ผู้ร่วมงาน / ผู้ร่วมวิจัย (EN):
คำสำคัญ (EN):
บทคัดย่อ (EN): Histamine fish poisoning becomes highly concern not only in public health but also economic aspect. Histamine is produced from histidine in fish muscles by bacterial decarboxylase enzyme. Several techniques have been developed to determine the level of histamine in fish and their products but the effective method for detecting histamine producing bacteria is still required. This study was attempted to detect histamine producing bacteria by newly developed PCR condition. Histamine producing bacteria were isolated from scombroid fish and determined the ability to produce histamine of isolated bacteria by biochemical and TLC assays. PCR method was developed to target the histidine decarboxylase gene (hdc). The result showed that fifteen histamine producing bacterial isolates and three standard strains produced an amplicon at the expected size of 571 bp after amplified by PCR using Hdc_2F/2R primers. Fifteen isolates of histamine producing bacteria were classified as M. morganii, E. aerogenes, and A. baumannii. The lowest detection levels of M. morganii and E. aerogenes were 102 and 105 Cfu/mL in culture media and 103 and 106 Cfu/mL in fish homogenates, respectively. The limit of detection by this method was clearly shown to be sensitive because the primers could detect the presence of M. morganii and E. aerogenes before the histamine level reached the regulation level at 50 ppm. Therefore, this PCR method exhibited the potential efficiency for detecting the hdc gene from histamine producing bacteria and could be used to prevent the proliferation of histamine producing bacteria in fish and fish products. © 2015, Association of Food Scientists & Technologists (India).
ปีเริ่มต้นงานวิจัย (EN): 2559
ปีสิ้นสุดงานวิจัย (EN): 2559
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84954398412&doi=10.1007%2fs13197-015-1982-1&partnerID=40&md5=59e5481633748bea1952786961a6e502
ลิขสิทธิ์ (EN): แสดงที่มา-ไม่ใช้เพื่อการค้า-ไม่ดัดแปลง 3.0 ประเทศไทย (CC BY-NC-ND 3.0 TH)
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
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Development of molecular approach based on PCR assay for detection of histamine producing bacteria
Wongsariya K.
มหาวิทยาลัยมหิดล
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