สืบค้นงานวิจัย
Multitarget quantitative PCR improves detection and predicts cultivability of the pathogen Burkholderia pseudomallei
Göhler A. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Multitarget quantitative PCR improves detection and predicts cultivability of the pathogen Burkholderia pseudomallei
ผู้แต่ง / หัวหน้าโครงการ (EN): Göhler A.
บทคัดย่อ (EN): Burkholderia pseudomallei is present in the environment in many parts of the world and causes the often-fatal disease melioidosis. The sensitive detection and quantification of B. pseudomallei in the environment are a prerequisite for assessing the risk of infection. We recently reported the direct detection of B. pseudomallei in soil samples using a quantitative PCR (qPCR) targeting a single type three secretion system 1 (TTSS1) gene. Here, we extend the qPCR-based analysis of B. pseudomallei in soil by validating novel qPCR gene targets selected from a comparative genomic analysis. Two hundred soil samples from two rice paddies in northeast Thailand were evaluated, of which 47% (94/200) were B. pseudomallei culture positive. The TTSS1 qPCR and two novel qPCR assays that targeted open reading frames (ORFs) BPSS0087 and BPSS0745 exhibited detection rates of 76.5% (153/200), 34.5% (69/200), and 74.5% (150/200), respectively. The combination of TTSS1 and BPSS0745 qPCR increased the detection rate to 90% (180/200). Combining the results of the three qPCR assays and the BPSS1187 nested PCR previously published, all 200 samples were positive by at least one PCR assay. Samples positive by either TTSS1 (n = 153) or BPSS0745 (n = 150) qPCR were more likely to be direct-culture positive, with odds ratios of 4.0 (95% confidence interval [CI], 1.7 to 9.5; P < 0.001) and 9.0 (95% CI, 3.1 to 26.4; P < 0.001), respectively. High B. pseudomallei genome equivalents correlated with high CFU counts by culture. In conclusion, multitarget qPCR improved the B. pseudomallei detection rate in soil samples and predicted culture positivity. This approach has the potential for use as a sensitive environmental screening method for B. pseudomallei. © 2017 American Society for Microbiology. All Rights Reserved.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016614207&doi=10.1128%2fAEM.03212-16&partnerID=40&md5=b15f59cc395663a8ae51fddb8b2ae701
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Type III Secretion Systems
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Göhler A.. "Multitarget quantitative PCR improves detection and predicts cultivability of the pathogen Burkholderia pseudomallei". ไม่ระบุหน่วยงาน. ม.ป.ป.

Göhler A.. "Multitarget quantitative PCR improves detection and predicts cultivability of the pathogen Burkholderia pseudomallei". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Multitarget quantitative PCR improves detection and predicts cultivability of the pathogen Burkholderia pseudomallei
Göhler A.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Analysis of the prevalence, secretion and function of a cell cycle-inhibiting factor in the melioidosis pathogen Burkholderia pseudomallei Immune response to recombinant Burkholderia pseudomallei FliC Soil nutrient depletion is associated with the presence of Burkholderia pseudomallei Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay Functionalized polyurethane applied for foodborne pathogen detection การตรวจหาเชื้อก่อโรคอุจจาระร่วงเฉียบพลัน 19 ชนิด ด้วยวิธี Quantitative real-time PCR Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia specie Development of Zika virus detection method by real time RT-PCR for using in Thailand The appearance of allelopathic substances affecting the presence of Burkholderia pseudomallei Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection
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