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Epr3 is a conserved immunogenic protein among Aeromonas species and able to induce antibody response in Nile tilapia
Peepim T. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Epr3 is a conserved immunogenic protein among Aeromonas species and able to induce antibody response in Nile tilapia
ผู้แต่ง / หัวหน้าโครงการ (EN): Peepim T.
บทคัดย่อ (EN): The present study retrospectively identified sixteen putative aeromonad isolates associated with previous disease outbreaks in farmed tilapia, jade perch, and striped catfish. Based on a combination of biochemical characterization and homology identity of 16S rDNA, the majority of the isolates were identified as Aeromonas caviae (n = 10) while the remaining isolates were Aeromonas veronii (n = 4), Aeromonas jandaei (n = 1) and Plesiomonas shigelloides (n = 1). Potential antigenic gene of epr3 from A. caviae isolates was identified and recombinant proteins were expressed in E. coli system for investigation of immunogenicity in vivo. The result indicated the β-sheet domain (comprising amino acid residue 23–171) expressed in a soluble form while the α-helix domain (residues 172–346) of Epr3 protein expressed in an insoluble form. Interestingly, all 15 identified Aeromonas strains naturally produced Epr3 evidenced by Western blot analysis detected with Epr3-specific polyclonal antibodies. Serum collected from the surviving fish after exposure to A. caviae AH15 was able to react with two domains of the recombinant Epr3. Two immunogenic domains of Epr3 had the capability of enhancing fish humoral immunity but a soluble form (β-sheet) induced a higher antibody response compared to an insoluble form (α-helix). A combination of two forms provoked the strongest antibody response as indicated by dot blot assay. Furthermore, Western blot results showed that sera from fish immunized with Epr3 β-sheet domain, α-helix domain, and pool of both domains could also react with specific protein bands from bacterial lysates of A. caviae, A. veronii and A. jandaei. The result suggests that the common immunogenic protein Epr3 is a promising candidate antigen for development of a cross-protective vaccine against different isolates of Aeromonas spp. Statement of relevance The authors strongly believe that our manuscript would provide significant knowledge to fish aquaculture especially to that of the tilapia (Oreochromis spp.) farming and vaccine development for Aeromonas spp. infection. © 2016 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979277011&doi=10.1016%2fj.aquaculture.2016.07.022&partnerID=40&md5=41fc6687bd1b2e4291f2dcc7ca36b7c6
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Tilapia
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Peepim T.. "Epr3 is a conserved immunogenic protein among Aeromonas species and able to induce antibody response in Nile tilapia". ไม่ระบุหน่วยงาน. ม.ป.ป.

Peepim T.. "Epr3 is a conserved immunogenic protein among Aeromonas species and able to induce antibody response in Nile tilapia". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Epr3 is a conserved immunogenic protein among Aeromonas species and able to induce antibody response in Nile tilapia
Peepim T.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.) HIV-1 envelope glycoproteins from diverse clades differentiate antibody responses and durability among vaccinees Naturally concurrent infections of bacterial and viral pathogens in disease outbreaks in cultured Nile tilapia (Oreochromis niloticus) farms Inverse association of plasma IgG antibody to Aggregatibacter actinomycetemcomitans and high C-reactive protein levels in patients with metabolic syndrome and periodontitis Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection of Fasciola gigantica circulating fatty acid binding protein Broad-spectrum monoclonal antibodies against chikungunya virus structural proteins: Promising candidates for antibody-based rapid diagnostic test development Influenza neuraminidase subtype N1: Immunobiological properties and functional assays for specific antibody response Antibody response to Zika and Dengue virus among the Zika realtime PCR positive cases in Thailand, 2016 Characterization of plasmodium vivax early transcribed membrane protein 11.2 and exported protein 1 Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models o
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