คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Sittiporn Parnmen

เผยแพร่โดย

1 Toxicology Center, National Institute of Health, Department of Medical Sciences, Ministry of Public Health 2 Regional Medical Sciences Center 8 Udonthani, Department of Medical Sciences, Ministry of Public Health

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งานวิจัยที่แนะนำ THE UTILIZATION OF CLIMATIC DATA TO SUPPORT AGRONOMIC PRACTISES IN RUBBER PLANTATION MOLECULAR CHARACTERIZATION OF SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) GENE FROM HEVEA BRASILIENSIS (MUELL. ARG.) Development of the Global Earthquake Model’s neotectonic fault database The Utilization of Natural Rubber as Raw Material for LPG Rubber Seal Compound Manufacturing Molecular identification of poisonous mushrooms using nuclear ITS region and peptide toxins: a retrospective study on fatal cases in Thailand DNA barcoding for identification of poisonous mushrooms from 187 cases of mycetism in Thailand Differences between Cryptococcus neoformans and Cryptococcus gattii in the molecular mechanisms governing utilization of D-amino acids as the sole nitrogen source Traditional practice of leaf litter harvesting and utilization by farmers in west-central Bhutan: Paving the way for sustainable management Serogroup, virulence, and molecular traits of Vibrio parahaemolyticus isolated from clinical and cockle sources in northeastern Thailand Molecular evolution of PvMSP3a block II in Plasmodium vivax from diverse geographic origins

คัดลอก URL

ชื่อเรื่อง (EN):	Molecular genetics database of poisonous mushrooms and sustainable utilization
ผู้แต่ง / หัวหน้าโครงการ (EN):	Sittiporn Parnmen
บทคัดย่อ (EN):	Cases of mushroom poisoning in local Thai populations have increased annually and continue to be a problem faced by health care professionals. A total of 5,082 cases with 27 deaths were reported to the Bureau of Epidemiology, Ministry of Public Health during 2014 to April 2018. In the laboratory, poisonous mushrooms are routinely identified based on morphology and toxic chemistry. However, the samples are generally not well preserved in for clinical diagnosis and standard methods for morphological identification do not always yield satisfactory results as well as standard reference material is expensive. Hence, the aims of this study were i) to construct a DNA database of nuclear ribosomal internal transcribed spacer (ITS) region, nuclear large-subunit (LSU) ribosomal DNA, DNA-directed RNA polymerase II subunit rpb1 (RPB1), Amatoxins-encoding gene (AMA) and Phallotoxins-encoding gene (PHA) and ii) to investigate the applicability of DNA markers as a DNA barcode for identifying the poisonous mushrooms and transfering knowledge-based prevention to communities. A total of 312 new DNA sequences of mushroom samples obtained from clinically reported cases and natural habitat were generated in this study. BLAST database with local sequences were constructed in Toxicology center as a references. Molecular barcoding confirmed the presence of new species and new records of several poisonous mushroom species, namely Amanita digitosa (Gastrointestinal syndrome), A. exitialis (Phalloides syndrome), A. gleocystidiosa (Phalloides syndrome), Cantharocybe virosa (Gastrointestinal syndrome)and Chlorophyllum globosum (Gastrointestinal syndrome). Four unidentified species belong to the genera Hygrocybe, Inocybe, Parvixerocomus and Tylopilus, which are responsible for gastrointestinal syndrome, muscarine syndrome and the later undefined species of Tylopilus and Parvixerocomus are responsible for a cause of unexpected death.These poisonous mushroom species provided new and informative data for clinical studies. Measures should be taken to prevent the consumption of toxic mushrooms.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=655
เผยแพร่โดย (EN):	1 Toxicology Center, National Institute of Health, Department of Medical Sciences, Ministry of Public Health 2 Regional Medical Sciences Center 8 Udonthani, Department of Medical Sciences, Ministry of Public Health
คำสำคัญ (EN):	Poisonous mushroom
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science