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Detection of DNA of leishmania siamensis in sergentomyia (Neophlebotomus) iyengari (Diptera: Psychodidae) and molecular identification of blood meals of sand flies in an affected area, Southern Thailand
Siripattanapipong S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Detection of DNA of leishmania siamensis in sergentomyia (Neophlebotomus) iyengari (Diptera: Psychodidae) and molecular identification of blood meals of sand flies in an affected area, Southern Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN): Siripattanapipong S.
บทคัดย่อ (EN): In Thailand, leishmaniasis is an emerging vector-borne disease that has become a public health concern. In related epidemiological surveys to identify potential Leishmania vectors in the affected areas, DNA of Leishmania martiniquensis (Kinetoplastida: Trypanosomatidae) was detected in Sergentomyia (Neophebotomus) gemmea (Diptera: Psychodidae) and Sergentomyia (Parrotomyia) barraudi (Diptera: Psychodidae). Recently, a more elaborate study was conducted in the same areas that included sand fly species identification, screening sand flies for the presence of Leishmania DNA and blood meal analysis to identify potential reservoir hosts directed toward assessing the risk of human infection. Twenty-nine archived pools of sand flies collected in Hat Samran District, Trang Province were used in this study. Sand fly species were confirmed using PCR encompassing regions within the mitochondrial DNA. Leishmania DNA was detected using PCR of the heat shock protein 70 region (hsp70-PCR) and blood meal identification was performed using PCR of the cyt b gene of vertebrate mitochondrial DNA (cytbnd1- PCR) and human-specific AluYb8 repeat (AluYb8-PCR). Four sand fly species were confirmed, i.e., Phlebotomus (Anaphlebotomus) stantoni (Diptera: Psychodidae), S. barraudi, Sergentomyia (Neophlebotomus) iyengari (Diptera: Psychodidae), and S. gemmea. Leishmania siamensis was detected in one female S. iyengari. Only human blood was detected in P. stantoni and S. gemmea, while both sun skink (Mabuya multifasciata) and human blood were detected in S. iyengari. In this study, we showed that S. iyengari could be a potential vector of L. siamensis infection among humans. © The Author(s) 2018.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056840350&doi=10.1093%2fjme%2ftjy069&partnerID=40&md5=d3963adc8709934348c6c52a0d50fdf1
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Thailand
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Detection of DNA of leishmania siamensis in sergentomyia (Neophlebotomus) iyengari (Diptera: Psychodidae) and molecular identification of blood meals of sand flies in an affected area, Southern Thailand
Siripattanapipong S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Detection of an Unknown Trypanosoma DNA in a Phlebotomus stantoni (Diptera: Psychodidae) Collected From Southern Thailand and Records of New Sand Flies With Reinstatement of Sergentomyia hivernus Rayn Molecular identification and genetic variation of hematophagous flies, (Diptera: Muscidae: Stomoxyinae) in Thailand based on cox1 barcodes Molecular detection and characterization of noroviruses in river water in Thailand Species composition and nocturnal activity of phlebotomine sand flies (Diptera: Psychodidae) inhabiting a limestone cave in Thailand First molecular identification of Strongyloides fuelleborni in long-tailed macaques in Thailand and Lao People's Democratic Republic reveals considerable genetic diversity DNA barcoding for identification of poisonous mushrooms from 187 cases of mycetism in Thailand Corrigendum to “Wing geometry of Phlebotomus stantoni and Sergentomyia hodgsoni from different geographical locations in Thailand” [C. R. Biologies 340(1) (2017) 37–46] (S1631069116301317)(10.1016/j.c Molecular detection of Rickettsia, Anaplasma, Coxiella and Francisella bacteria in ticks collected from Artiodactyla in Thailand DNA-based identification of gastrointestinal irritant mushrooms in the genus Chlorophyllum: a food poisoning case in Thailand Detection, identification and characterization of enteric bacterial pathogens in a case-control study of acute diarrhea in Thailand
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