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Molecular Analysis of Botryodiplodia theobromae Isolates from Rubber in Vietnam using rDNAv ITS Sequencing and ISSR Markers
Nguyen Anh Nghia - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Molecular Analysis of Botryodiplodia theobromae Isolates from Rubber in Vietnam using rDNAv ITS Sequencing and ISSR Markers
ผู้แต่ง / หัวหน้าโครงการ (EN): Nguyen Anh Nghia
บทคัดย่อ (EN): A total of 20 isolates of Botryodiplodia theobromae from different Hevea clones growing in various rubber plantations in Vietnam was analysed using Ribosomal DNA ITS (rDNA ITS) sequencing and Inter Simple Sequence Repeat (ISSR) markers. Analysis of rDNA-ITS sequences revealed that 20 studied isolates have the same size (541 bp) and these sequences are identical. BLAST search showed that the rDNA – ITS sequences of the studied isolates was 98 – 99% similarity to that of rDNA – ITS of B. theobromae deposited in GenBank. The identical sequences in rDNA-ITS regionimplied high conservation of this region in the genome of the studied isolates. ISSR analysis using 16 primers produced a total of 214 DNA bands, of which the polymorphic DNA bands was accounted for 76.6%. The phylogenetic tree produced from UPGMAS analysis based on Nei and Li coefficient divided 20 isolates into four genetic clusters. Analysis of these clusters showed the relationship among isolates from different regions and no relationship between genetic groups and the host origins (rubber clones). Some unique isolates that need further investigation.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): Ribosomal DNA ITS
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Molecular Analysis of Botryodiplodia theobromae Isolates from Rubber in Vietnam using rDNAv ITS Sequencing and ISSR Markers
Nguyen Anh Nghia
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
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