สืบค้นงานวิจัย
Molecular evolution and functional characterization of a bifunctional decarboxylase involved in lycopodium alkaloid biosynthesis
Bunsupa S. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Molecular evolution and functional characterization of a bifunctional decarboxylase involved in lycopodium alkaloid biosynthesis
ผู้แต่ง / หัวหน้าโครงการ (EN): Bunsupa S.
บทคัดย่อ (EN): Lycopodium alkaloids (LAs) are derived from lysine (Lys) and are found mainly in Huperziaceae and Lycopodiaceae. LAs are potentially useful against Alzheimer’s disease, schizophrenia, and myasthenia gravis. Here, we cloned the bifunctional lysine/ornithine decarboxylase (L/ODC), the first gene involved in LA biosynthesis, from the LA-producing plants Lycopodium clavatum and Huperzia serrata. We describe the in vitro and in vivo functional characterization of the L. clavatum L/ODC (LcL/ODC). The recombinant LcL/ODC preferentially catalyzed the decarboxylation of L-Lys over L-ornithine (L-Orn) by about 5 times. Transient expression of LcL/ODC fused with the amino or carboxyl terminus of green fluorescent protein, in onion (Allium cepa) epidermal cells and Nicotiana benthamiana leaves, showed LcL/ODC localization in the cytosol. Transgenic tobacco (Nicotiana tabacum) hairy roots and Arabidopsis (Arabidopsis thaliana) plants expressing LcL/ODC enhanced the production of a Lys-derived alkaloid, anabasine, and cadaverine, respectively, thus, confirming the function of LcL/ODC in plants. In addition, we present an example of the convergent evolution of plant Lys decarboxylase that resulted in the production of Lys-derived alkaloids in Leguminosae (legumes) and Lycopodiaceae (clubmosses). This convergent evolution event probably occurred via the promiscuous functions of the ancestral Orn decarboxylase, which is an enzyme involved in the primary metabolism of polyamine. The positive selection sites were detected by statistical analyses using phylogenetic trees and were confirmed by site-directed mutagenesis, suggesting the importance of those sites in granting the promiscuous function to Lys decarboxylase while retaining the ancestral Orn decarboxylase function. This study contributes to a better understanding of LA biosynthesis and the molecular evolution of plant Lys decarboxylase. © 2016 American Society of Plant Biologists. All rights reserved.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84982915231&doi=10.1104%2fpp.16.00639&partnerID=40&md5=259d5834942f5e0f9e45055d836ff2b4
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Tobacco
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
หากไม่พบเอกสารฉบับเต็ม (Full Text) โปรดติดต่อหน่วยงานเจ้าของข้อมูล

การอ้างอิง

Bunsupa S.. "Molecular evolution and functional characterization of a bifunctional decarboxylase involved in lycopodium alkaloid biosynthesis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Bunsupa S.. "Molecular evolution and functional characterization of a bifunctional decarboxylase involved in lycopodium alkaloid biosynthesis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



TARR Wordcloud:
Molecular evolution and functional characterization of a bifunctional decarboxylase involved in lycopodium alkaloid biosynthesis
Bunsupa S.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Molecular and functional characterization of vacuolar-ATPase from the American dog tick Dermacentor variabilis Molecular cloning and characterization of Mj-mov-10, a putative RNA helicase involved in RNAi of kuruma shrimp Molecular detection and characterization of noroviruses in river water in Thailand Molecular cloning and characterization of Fasciola gigantica thioredoxin-glutathione reductase Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii Molecular isolation and characterization of a haemocyanin of Macrobrachium rosenbergii reveal its antibacterial activities Molecular characterization of serine protease inhibitor isoform 3, SmSPI, from Schistosoma mansoni Association of a Viroid with Tapping Panel Dryness of Hevea brasiliensis and its Molecular Characterization Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus
คัดลอก URL
กระทู้ของฉัน
ผลการสืบค้นทั้งหมด โพสต์     เรียงลำดับจาก
พัฒนาระบบโดยศูนย์ความรู้เฉพาะด้านวิศวกรรมความรู้และวิศวกรรมภาษาDeveloped by Center of Excellence for Unified Knowledge and Language Engineering
สนับสนุนโดยสำนักงานพัฒนาการวิจัยการเกษตร (องค์การมหาชน)Supported by the Agricultural Research Development Agency (Public Organization)
แบบสำรวจความพึงพอใจQuestionair