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Production of river buffalo (Bubalus bubalis) calves by embryo in vitro production-vitrification and transfer techniques in the Philippines (Buffalo Bulletin (Thailand),36(4))
Cruz, Libertado C. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Production of river buffalo (Bubalus bubalis) calves by embryo in vitro production-vitrification and transfer techniques in the Philippines (Buffalo Bulletin (Thailand),36(4))
ผู้แต่ง / หัวหน้าโครงการ (EN): Cruz, Libertado C.
หน่วยงานสังกัดผู้แต่ง (EN): Nueva Ecija (Philippines). Reproductive Biotechnology Laboratory
บทคัดย่อ (EN): Aimed at producing genetically superior water buffaloes for increased milk and meat purposes, the Philippines through the Philippine Carabao Center endeavoured to develop the advance reproductive biotechnologies. The efficiency of the in vitro embryo production in the production of genetically superior animal was assessed. In Study 1, the viability of in vitro derived vitrified embryos (2n=50) were assessed by non-surgical transfer to river (2n=50) recipients. Study 2 examined the efficiency of swamp buffloes (2n=48) as surrogate mothers of river buffalo embryos, and Study 3 examined the possibility of twinning in water buffaloes by embryo transfer. A Satellite Embryo Biotechnology Laboratory was established in India to secure ovaries from slaughtered river buffaloes and oocytes were collected, matured and fertilized in vitro and cultured for embryo development. In vitro fertilization was carried out using top three progeny tested bulls from National Dairy Development Board of India. Resultant embryos were cryopreserved by vitrification procedure and transported to the Philippines in liquid nitrogen for embryo transfer. Results in Study 1 demonstrated 16.36% (9/55) pregnancy rate and 10.91% (6/55) calving rate. In Study 2, 12.5% (5/40) pregnancy and 10% (4/40) calving rate were achieved and 23.1% (6/26) calving rate was achieved in study 3 with 3.8% (1/26) twinning rate when embryos are transferred in twos. The results demonstrated efficiency of the technology and its potential application in water buffalo genetic improvement program.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/374167
คำสำคัญ (EN): Philippines
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Production of river buffalo (Bubalus bubalis) calves by embryo in vitro production-vitrification and transfer techniques in the Philippines (Buffalo Bulletin (Thailand),36(4))
Cruz, Libertado C.
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Production of buffalo milk (Bubalus bubalis) in Brazil (Buffalo Bulletin,39(3)) Congenital anomalies of the uterus in riverine buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(4)) Anatomy of the carpal articulation of buffalo calves (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(4)) Cryopreservation of buffalo (Bubalus bubalis) semen-limitations and expectations (Buffalo Bulletin (Thailand),36(1)) DGAT1 gene polymorphisms and its association with milk production traits in Mehsana buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(2)) Anatomy of the femorotibial joint of stifle of buffalo calves (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(4)) Clinico-therapeutic management of nervous ketosis in Jaffarabadi buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(3)) Production performance of Nagpuri buffalo under field condition (Buffalo Bulletin (Thailand),35(4)) Ovarian teratoma in a water buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),35(4)) Molecular characterization of exon 2-3 of majorhistocompatibility complex (MHC) class I in buffalo (Bubalus bubalis) (Buffalo Bulletin (Thailand),36(3))
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