สืบค้นงานวิจัย
A comparison of virus concentration methods for molecular detection and characterization of rotavirus in bivalve shellfish species
Kittigul L. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): A comparison of virus concentration methods for molecular detection and characterization of rotavirus in bivalve shellfish species
ผู้แต่ง / หัวหน้าโครงการ (EN): Kittigul L.
บทคัดย่อ (EN): The objectives of this study were to develop a method for concentrating rotavirus, to assess the detection rate, and to characterize the genotype of naturally occurring rotavirus in bivalve shellfish species; including oysters (Saccostrea forskali), cockles (Anadara nodifera), and mussels (Perna viridis). The results demonstrated that an adsorption-twice elution-extraction method was less-time consuming method of concentrating the spiked rotavirus, yielding high sensitivity of 1.14 genome copies/g of digestive tissues from all three shellfish species, as detected using an RT-nested PCR. In seeding experiments, rotavirus as low as 1.39 genome copies was able to be detected in 4g of digestive tissues or per sample. In the period of August 2011 to July 2012, of the 300 bivalve shellfish samples collected and tested, 24 (8.0%) were found to be contaminated with rotavirus, the figures being: oysters, 13/100 samples; mussels, 10/100 samples; and cockles, 1/100 samples. By DNA sequencing of the RT-nested PCR products and phylogenetic analysis, the rotaviruses detected were classified into G1, lineage II (4 samples); G3 (10 samples): lineage I (3 samples), lineage IIIc (3 samples), lineage IIId (3 samples), lineage IV (1 sample); G9 (6 samples); and G12, lineage III (1 sample). These findings suggest that this virus concentration method provides high sensitivity for the detection of rotavirus from the three bivalve shellfish species. The prevalence of rotavirus and the identified genotypes contribute to the molecular epidemiology of rotavirus in different shellfish species. © 2014 Elsevier Ltd.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84906509932&doi=10.1016%2fj.fm.2014.07.020&partnerID=40&md5=1db8ae05a6dc2814e5c8c19fbe90c81f
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Shellfish
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Kittigul L.. "A comparison of virus concentration methods for molecular detection and characterization of rotavirus in bivalve shellfish species". ไม่ระบุหน่วยงาน. ม.ป.ป.

Kittigul L.. "A comparison of virus concentration methods for molecular detection and characterization of rotavirus in bivalve shellfish species". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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A comparison of virus concentration methods for molecular detection and characterization of rotavirus in bivalve shellfish species
Kittigul L.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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