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Distinctive histopathology of Spiroplasma eriocheiris infection in the giant river prawn Macrobrachium rosenbergii
Srisala J. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Distinctive histopathology of Spiroplasma eriocheiris infection in the giant river prawn Macrobrachium rosenbergii
ผู้แต่ง / หัวหน้าโครงการ (EN): Srisala J.
บทคัดย่อ (EN): Spiroplasma eriocheiris was first named as the cause of tremor disease in the Chinese mitten crab Eriocheir sinensis in China in 2003. It was subsequently reported from China to infect also the crayfish Procambarus clarkii, the giant river prawn Macrobrachium rosenbergii, the oriental prawn Macrobrachium nipponensis and the whiteleg shrimp Penaeus vannamei. Unfortunately, none of these publications give clear pictures of the histopathology caused by the pathogen, even though this is one of the first diagnostic procedures often performed for newly reported diseases. Here we describe distinctive histological lesions caused in the hepatopancreatic tissue of M. rosenbergii infected with S. eriocheiris. In the hepatopancreas (HP), these consist of basophilic or mixed basophilic/eosinophilic lesions in cells confined to the interstitial spaces of the HP and not involving the hepatopancreatic tubule epithelial cells. Examination of the lesions by transmission electron microscopy revealed the presence of colonies of intracellular, un-walled bacterial cells of variable shape (but many crescent shaped) and approximately 150 nm in diameter. PCR using group-specific primers for the SSU rRNA followed by cloning and sequencing revealed 99.9% sequence identity to the matching sequence of S. eriocheiris. PCR tests for presence of S. eriocheiris in hepatopancreatic tissue samples gave positive test results, and in situ hybridization assays using a probe derived from the PCR product gave positive signals in the interstitial spaces of the HP, corresponding to the regions of the distinctive histological lesions. Less severe lesions containing cells with eosinophilic/basophilic inclusions were also found and confirmed by in situ hybridization in heart muscle, skeletal muscle, gills, connective tissue and the ventral nerve cord. Recognition of the distinctive histological lesions in interstitial spaces of the hepatopancreatic tissue of M. rosenbergii by histological analysis can be used as a strong presumptive indication of infection by S. eriocheiris and may help in the control of this rapidly-spreading, emerging pathogen. © 2018 Elsevier B.V.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046832504&doi=10.1016%2fj.aquaculture.2018.04.057&partnerID=40&md5=0a05bc42d53bdd9a9c5597e491df900c
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Spiroplasma
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Distinctive histopathology of Spiroplasma eriocheiris infection in the giant river prawn Macrobrachium rosenbergii
Srisala J.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Identification and characterization of a crustacean female sex hormone in the giant freshwater prawn, Macrobrachium rosenbergii The existence of kisspeptin-like peptides and effects on ovarian development and maturation in the giant freshwater prawn Macrobrachium rosenbergii Spermatophore affects the egg-spawning and egg-carrying behavior in the female giant freshwater prawn, Macrobrachium rosenbergii Neuropeptide F stimulates ovarian development and spawning in the female giant freshwater prawn, Macrobrachium rosenbergii, and its expression in the ovary during ovarian maturation cycle Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol Interaction study of a novel Macrobrachium rosenbergii effector caspase with B2 and capsid proteins of M.rosenbergii nodavirus reveals their roles in apoptosis ผลของอุณหภูมิและความหนาแน่นของการลำเลียงลูกกุ้งขาว (Litopenaeus vannamei) ลูกกุ้งกุลาดำ (Penaeus monodon) และกุ้งก้ามกราม (Macrobrachium rosenbergii) ระยะโพสลาวา ต่อการรอดตาย ความแข็งแรงและการเปลี่ยนแ Molecular isolation and characterization of a haemocyanin of Macrobrachium rosenbergii reveal its antibacterial activities การขนส่งกุ้งก้ามกราม (Macrobrachium rosenbergii) มีชีวิตโดยใช้วัสดุมีความชื้น In silico neuropeptidome of female Macrobrachium rosenbergii based on transcriptome and peptide mining of eyestalk, central nervous system and ovary
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