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Rapid Molecular Identification and Detection of Corynespora cassiicola of Hevea brasiliensis
Xianbao Liu - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Rapid Molecular Identification and Detection of Corynespora cassiicola of Hevea brasiliensis
ผู้แต่ง / หัวหน้าโครงการ (EN): Xianbao Liu
บทคัดย่อ (EN): Corynespora leaf fall disease (CLFD) of Hevea brasiliensis is caused by the fungus Corynespora cassiicola. This disease has emerged as a major constraint for natural rubber production over the last two decades. Eighteen strains of C. cassiicola from China were used in this study. Genomic DNAs were extracted and the ribosomal DNA internal transcribed spacer (ITS) regions were amplified and sequenced. The results showed only small variances in base sequences. Species-specific PCR primers CorP1 and CorP2 for C. cassiicola were designed after multiple sequence alignment of C. cassiicola against other species from the Corynespora genus. A single fragment approximately 300 bp in length was amplified from DNA from isolates of C. cassiicola. No product was amplified with DNA from the other 18 fungi or from DNA of rubber tree leaf tissue. The detection limit for C. cassiicola genomic DNA was 0.1 pg using species-specific primers.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): PCR assay
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Rapid Molecular Identification and Detection of Corynespora cassiicola of Hevea brasiliensis
Xianbao Liu
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Surveillance on Corynespora Leaf Fall Disease: Incidence and Severity on Natural Rubber (Hevea brasiliensis) in Certain Hot Spots Areas in Kerala CDNA –AFLP ANALYSIS REVEALS DIFFERENTIAL GENE EXPRESSION IN RUBBER TREE (HEVEA BRASILIENSIS) INOCULATED WITH CORYNESPORA CASSIICOLA PATHOGEN MORPHOLGICAL ANALYSIS OF CORYNESPORA CASSIICOLA ISOLATES FROM HEVEA PLANTATIONS IN MALAYSIA Differential Expression Analysis by cDNA-AFLP of Hevea brasiliensis after Inoculation with the Pathogen Corynespora cassiicola Identification of Resistance of Hevea brasiliensis Clones to Corynespora Leaf Fall Disease Differentiating Corynespora Cassiicola Isolated From Hevea Plantations in Malaysia Using Inter Simple Sequence Repeat (ISSR) Markers Caffeic Acid O-Methyltransferase (COMT) Gene of the Pheny1 Propanoid Pathway Involved in Resistance to Corynespora Leaf Disease in Rubber (Hevea brasiliensis) Generation of Tranceriptome Recources in Rubber (Hevea brasiliensis) in Response to Corynespora Cassiicola Causing Corynespora Leaf Disease for Gene Discovery and Marker Identification Using Ngs Platf Identification and Molecular Characterization of Multiple forms of â-1. 3-Glucanade Gene Promoter from Hevea brasiliensis through Inverse PCR IDENTIFICATION OF DROUGHT TOLERANT GENES BY QUANTITATIVE EXPRESSION ANALYSIS IN HEVEA BRASILIENSIS
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