คลังข้อมูลการวิจัยการเกษตรไทย

ผู้แต่ง Jakkrawarn Chompoosri

เผยแพร่โดย

1 National Institute of Health, Department of Medical Sciences, Nonthaburi 2 Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

เผยแพร่เมื่อ ไม่ระบุวันที่เผยแพร่

หน่วยงาน ไม่ระบุหน่วยงาน

งานวิจัยที่แนะนำ Group B Streptococcal bacteremia burden and trends over time, 2007-15, Thailand Climatic factors influencing dengue hemorrhagic fever in Kolaka district, Indonesia Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand Wolbachia supergroups A and B in natural populations of medically important filth flies (Diptera : Muscidae, Calliphoridae, and Sarcophagidae) in Thailand Efficacy of essential oils from Thai medicinal plants against insecticide-resistant Aedes aegypti mosquito from 3 dengue-risk northern provinces in Thailand ประสิทธิภาพของน้ำมันหอมระเหยจากพืชสมุนไพรไทยในการกำจัดยุงลายดื้อยา Efficacy of essential oils from Thai medicinal plants against insecticide-resistant Aedes aegypti mosquito Induction of defensin response to dengue infection in Aedes aegypti Insecticide susceptibility characterization and efficacy of insecticides for thermal space spray application against insecticide-resistant Aedes aegypti (L.) from Chanthaburi Province Effectiveness of eight-essential oils against the housefly, Musca domestica Prevalence of zoonotic Bartonella species among rodents and shrews in Thailand

คัดลอก URL

ชื่อเรื่อง (EN):	Efficacy of essential oils from Thai medicinal plants against insecticide-resistant Aedes aegypti mosquito in dengue-risk areas in Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN):	Jakkrawarn Chompoosri
บทคัดย่อ (EN):	Background: The Aedes aegypti mosquito is the primary vector of dengue fever and zika fever. Dengue fever is caused by one of the four closely related dengue viruses named DEN-1, DEN-2, DEN-3 and DEN-4, whereas zika fever is an infectious disease caused by the zika virus. In Thailand, the diseases have become major public health problems although there is currently dengue vaccine named Dengvaxia available. However, the vaccine has certain limitations of the efficacy to reduce dengue cases overall by approximately 65 percent and of the persons recommended by age 9–45 years. Besides, it has not yet been included as part of the National Immunisation Programme because of potential costs. In addition, the presence of insecticide resistance in Ae. aegypti populations has been reported in several provinces of Thailand. These reasons have led to ineffective control and prevention of the diseases. The present research pays attention to the essential oils from Thai medicinal plants which are non-toxic to humans and environment for testing against insecticide-resistant Ae. aegypti mosquito. The most effective essential oil will be further developed as a powerful tool for mosquito management and might be used instead of the chemical insecticides in the mosquito control program. Objective: This study aimed to determine the efficacy of five essential oils from Thai medicinal plants against insecticideresistant Ae. aegypti mosquito from 3 dengue-risk provinces in Thailand. Methods: Three northern provinces: Chiang Mai (Hang Dong and San Sai), Tak (Mueang and Ban Tak) and Pitsanulok (Mueang and Nakhon Thai) were chosen for the collection of Ae. aegypti larvae in 2015 based on forecast of dengue outbreak. The mosquito larvae were reared to adults in laboratory. F1 progeny of unfed female aged 3-5 days was tested for resistance status to five pyrethroid insecticides used currently for mosquito control: 0.15% Cyfluthrin, 0.22% Cypermethrin, 0.05% Deltamethrin, 0.05% Lambda-cyhalothrin and 0.75% Permethrin by the standard WHO susceptibility test. Five Thai medicinal plants: Citrus hystrix, Murraya siamensis, Ocimum basilicum, Ocimum sanctum and Piper betel were extracted by hydrodistillation for essential oils. Each essential oil was then prepared in concentration of 0.25%, 0.5%, 1.0%, 2.0%, 4.0% and 8.0%. Those were tested for mosquitocidal activity against insecticide-resistant Ae. aegypti mosquito by modification of the standard WHO susceptibility test. The mortality rates of mosquitoes were analyzed and compared by one-way ANOVA and Bonferroni’s multiple comparison tests. Results: The insecticide susceptibility test of Ae. aegypti revealed that the mosquitoes from those provinces were resistant to all five pyrethroids with the mortality rates of less than 80% according to WHO standard criteria. The essential oil susceptibility test of the insecticide resistant Ae. aegypti mosquito showed that M. siamensis, O.basilicum, C. hystrix, Piper betel and O. Sanctum provided mosquitocidal activity against Ae. aegypti from all three provinces with the significant mortality rates of 100%, 100%, 100%, 99-100% and 98-100% at minimal concentration of 2.0%, 4.0%, 8.0%, 8.0% and 8.0% at 24 hours, respectively (P<0.05). Conclusion: This study revealed that the Ae. aegypti mosquito from three study provinces was resistant to five pyrethroid insecticides. The essential oil from M. siamensis provided significantly highest efficacy against insecticideresistant Ae. aegypti mosquito from all three provinces, followed by O. basilicum, C. hystrix, P. betel and O. sanctum. The knowledge obtained from this study will be used to develop a product of anti-mosquito spray and transferred to public for mosquito management and for effective prevention of the Aedes mosquito-borne diseases.
บทคัดย่อ:	ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN):	en
เอกสารแนบ (EN):	http://nih.dmsc.moph.go.th/research/showimgdetil.php?id=648
เผยแพร่โดย (EN):	1 National Institute of Health, Department of Medical Sciences, Nonthaburi 2 Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
คำสำคัญ (EN):	Aedes mosquitoborne diseases
เจ้าของลิขสิทธิ์ (EN):	National Institute of Health, Department of Medical Science