สืบค้นงานวิจัย
Validation of the quantitative point-of-care CareStart biosensor for assessment of G6PD activity in venous blood
Bancone G. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Validation of the quantitative point-of-care CareStart biosensor for assessment of G6PD activity in venous blood
ผู้แต่ง / หัวหน้าโครงการ (EN): Bancone G.
บทคัดย่อ (EN): Introduction Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzymopathy in the human population affecting an estimated 8% of the world population, especially those living in areas of past and present malaria endemicity. Decreased G6PD enzymatic activity is associated with drug-induced hemolysis and increased risk of severe neonatal hyperbilirubinemia leading to brain damage. The G6PD gene is on the X chromosome therefore mutations cause enzymatic deficiency in hemizygote males and homozygote females while the majority of heterozygous females have an intermediate activity (between 30–80% of normal) with a large distribution into the range of deficiency and normality. Current G6PD qualitative tests are unable to diagnose G6PD intermediate activities which could hinder wide use of 8-aminoquinolines for Plasmodium vivax elimination. The aim of the study was to assess the diagnostic performances of the new Carestart G6PD quantitative biosensor. Methods A total of 150 samples of venous blood with G6PD deficient, intermediate and normal phenotypes were collected among healthy volunteers living along the north-western Thailand-Myanmar border. Samples were analyzed by complete blood count, by gold standard spectrophotometric assay using Trinity kits and by the latest model of Carestart G6PD biosensor which analyzes both G6PD and hemoglobin. Results Bland-Altman comparison of the CareStart normalized G6PD values to that of the gold standard assay showed a strong bias in values resulting in poor area under-the-curve values for both 30% and 80% thresholds. Performing a receiver operator curve identified threshold values for the CareStart product equivalent to the 30% and 80% gold standard values with good sensitivity and specificity values, 100% and 92% (for 30% G6PD activity) and 92% and 94% (for 80% activity) respectively. Conclusion The Carestart G6PD biosensor represents a significant improvement for quantitative diagnosis of G6PD deficiency over previous versions. Further improvements and validation studies are required to assess its utility for informing radical cure decisions in malaria endemic settings. © 2018 Bancone et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046647964&doi=10.1371%2fjournal.pone.0196716&partnerID=40&md5=2c1ddfcd5454f77cecd6a32afcedbc8c
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Spectrophotometry, Ultraviolet
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Bancone G.. "Validation of the quantitative point-of-care CareStart biosensor for assessment of G6PD activity in venous blood". ไม่ระบุหน่วยงาน. ม.ป.ป.

Bancone G.. "Validation of the quantitative point-of-care CareStart biosensor for assessment of G6PD activity in venous blood". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Validation of the quantitative point-of-care CareStart biosensor for assessment of G6PD activity in venous blood
Bancone G.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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