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Evaluation of a multiplex PCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in ground beef marketed in Marajo island (Buffalo Bulletin,41(4))
Carina Martins de Moraes - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Evaluation of a multiplex PCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in ground beef marketed in Marajo island (Buffalo Bulletin,41(4))
ผู้แต่ง / หัวหน้าโครงการ (EN): Carina Martins de Moraes
หน่วยงานสังกัดผู้แต่ง (EN): Castanhal (Brazil). School of Veterinary Medicine and Graduate Program in Animal Health in the Amazon. Food Hygiene and Quality Laboratory and Microbiology Laboratory
บทคัดย่อ (EN): The present work intends to use a mPCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in samples commercially available on Marajo Island, Para State, Brazil. For this purpose, a mPCR reaction with different concentrations of DNA and primers with different annealing temperatures was optimized. Next, 28 samples of ground meat marketed as being of bovine origin were collected and a mPCR for the determination of species and for the detection of Salmonella spp. were realized. The results demonstrated the efficiency of the proposed technique for identification of Bos taurus, Bubalus bubalis and Salmonella spp. We conclude that the proposed mPCR technique is efficient and that buffalo meat is marketed as bovine on the Island of Marajo and that such frauds violating the current legislation that normalizes the commercialization of ground beef.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/422459
คำสำคัญ (EN): Bovine
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Evaluation of a multiplex PCR for simultaneous detection of Bos taurus, Bubalus bubalis and Salmonella spp. in ground beef marketed in Marajo island (Buffalo Bulletin,41(4))
Carina Martins de Moraes
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Detection of Theileria and Anaplasma spp. in carrier buffaloes (Bubalus bubalis) by polymerase chain reaction in Tamil Nadu, India (Buffalo Bulletin,41(4)) The Population structure analysis in Sumbawa buffaloes (Bubalus bubalis) of Indonesia (Buffalo Bulletin,41(3)) Seasonal variation in semen quality and conception rate of Jaffarabadi buffalo bulls (Bubalus bubalis) in India (Buffalo Bulletin,41(3)) Arterial thrombosis and fibrinoid necrosis-possible etiology behind hydroallantois in Bubalus bubalis (Water buffalo) (Buffalo Bulletin,41(1)) The fertility of buffalo sperm (Bubalus bubalis) on three diluent types and three equilibration times (Buffalo Bulletin,41(3)) Morphological features of superficial and deep digital flexor tendons of forelimb in buffalo bull (Bubalus bubalis) in post-natal stages (Buffalo Bulletin,41(1)) Quality evaluation and safety assessment of buffalo milk collected from Mathura city (Buffalo Bulletin,41(3)) Assaying the concentration of immunoglobulin G in colostrum from females postpartum and serum from neonatal calves of buffaloes (Bubalus bubalis) (Buffalo Bulletin,41(3)) Etiology of diarrhoea among adult buffaloes (Bubalus bubalis) and their impact on haemato-biochemical and mineral profile (Buffalo Bulletin,41(4)) Modulating feed digestion and methane production by eucalyptus (Eucalyptus citriodora) leaves essential oils in water buffalo (Bubalus bubalis) (Buffalo Bulletin,41(1))
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