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Nanocontainer designed from an infectious hypodermal and hematopoietic necrosis virus (IHHNV) has excellent physical stability and ability to deliver shrimp tissues
Kiatmetha P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Nanocontainer designed from an infectious hypodermal and hematopoietic necrosis virus (IHHNV) has excellent physical stability and ability to deliver shrimp tissues
ผู้แต่ง / หัวหน้าโครงการ (EN): Kiatmetha P.
บทคัดย่อ (EN): A virus-like particle (VLP) is an excellent tool for a compound delivery system due to its simple composition, symmetrical structure and self-assembly. Its surface modification both chemically and genetically is established, leading to the target-specific delivery and improved encapsulation efficiency. However, its physical stabilities against many harsh conditions that guarantee long term storage and oral administration have been much less studied. Methods: IHHNV-VLPs were reconstructed from recombinant IHHNV capsid protein in E. coli. Their physical properties against three strong physical conditions including long term storage (0-30 days) in 4 °C, physical stabilities against broad ranged pH (4-9) and against three types of digestive enzymes were tested. Disassembly and reassembly of VLPs for encapsidating an enhanced green fluorescent protein tagged plasmid DNA (EGFP-VLPs) were controlled by the use of reducing agent (DTT) and calcium specific chelating agent (EGTA). Lastly, delivering ability of EGFP-VLPs was performed in vivo by intramuscular injection and traced the expression of GFP in the shrimp tissues 24 hr post-injection. Results: Upon its purification, IHHNV-VLPs were able to be kept at 4 °C up to 30 days with only slight degradation. They were very stable in basic condition (pH 8-9) and to a lesser extent in acidic condition (pH 4-6) while they could stand digestions of trypsin and chymotrypsin better than pepsin. As similar with many other non-enveloped viruses, the assembly of IHHNV-VLPs was dependent on both disulfide bridging and calcium ions which allowed us to control disassembly and reassembly of these VLPs to pack EGFP plasmid DNA. IHHNV-VLPs could deliver EGFP plasmids into shrimp muscles and gills as evident by RT-PCR and confocal microscopy demonstrating the expression of GFP in the targeted tissues. Discussion: There are extensive data in which capsid proteins of the non-enveloped viruses in the form of VLPs are constructed and used as nano-containers for therapeutic compound delivery. However, the bottleneck of its application as an excellent delivery container for oral administration would rely solely on physical stability and interacting ability of VLPs to the host cells. These properties are retained for IHHNV-VLPs reported herein. Thus, IHHNV-VLPs would stand as a good applicable nanocontainer to carry therapeutic agents towards the targeting tissues against ionic and digestive conditions via oral administration in aquaculture field. © Copyright 2018 Kiatmetha et al.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85059151573&doi=10.7717%2fpeerj.6079&partnerID=40&md5=f9e8b1c805880ca6f5809a8a647b339f
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): VLP
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Kiatmetha P.. "Nanocontainer designed from an infectious hypodermal and hematopoietic necrosis virus (IHHNV) has excellent physical stability and ability to deliver shrimp tissues". ไม่ระบุหน่วยงาน. ม.ป.ป.

Kiatmetha P.. "Nanocontainer designed from an infectious hypodermal and hematopoietic necrosis virus (IHHNV) has excellent physical stability and ability to deliver shrimp tissues". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Nanocontainer designed from an infectious hypodermal and hematopoietic necrosis virus (IHHNV) has excellent physical stability and ability to deliver shrimp tissues
Kiatmetha P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Infectious hypodermal and hematopoietic necrosis virus-like particles encapsulating VP28 double-stranded RNA protect shrimp from white spot syndrome virus In situ DIG-labeling, loop-mediated DNA Amplification (ISDL) for highly sensitive detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) การวิเคราะห์จีโนมของกุ้งกุลาดำโดยใช้เทคนิค southern blot hybridization เพื่อประโยชน์ในการวินิจฉัยโรค infectious hypodermal and hematopoietic necrosis virus (IHHNV) Sensitivity improvement of immunochromatographic strip test for infectious myonecrosis virus detection Testing of a pond-side molecular diagnostic tool for the detection of white spot syndrome virus in shrimp aquaculture การพัฒนาวัคซีนต่อต้านการติดเชื้อ nervous necrosis virus ในปลากะรัง ด้วยยีสต์อาร์มมิ่งเทคโนโลยี Use of microalgae Chlamydomonas reinhardtii for production of double-stranded RNA against shrimp virus การสร้าง infectious clones ของไวรัส Infectious myonecrosis virus (IMNV) และการผลิตไวรัส IMNV ของกุ้ง ในเซลล์แมลงเพาะเลี้ยงโดยใช้ Baculovirus vector เป็นตัวช่วย Infectious spleen and kidney necrosis disease (ISKND) outbreaks in farmed barramundi (Lates calcarifer) in Vietnam RT-LAMP detection of shrimp Taura syndrome virus (TSV) by combination with a nanogold-oligo probe
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