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Overexpression of the gibberellin 20-oxidase gene from Torenia fournieri resulted in modified trichome formation and terpenoid metabolities of Artemisia annua
Inthima P. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Overexpression of the gibberellin 20-oxidase gene from Torenia fournieri resulted in modified trichome formation and terpenoid metabolities of Artemisia annua
ผู้แต่ง / หัวหน้าโครงการ (EN): Inthima P.
บทคัดย่อ (EN): Gibberellins (GAs) are diterpenoid hormones, control various physiological developments in plants. The role of gibberellins on morphology and secondary metabolite production was examined in Artemisia annua, a medicinal plant that has been acknowledged as a source of artemisinin, an antimalarial compound. Subsequently, the GA20ox gene from Torenia fournieri (TfGA20ox2) was transferred to A. annua by Agrobacterium-mediated transformation. Compared with wild type plants, all nine transgenic plants showed significantly higher plant heights and artemisinin contents. The highest artemisinin content and yield in TfGA20ox2-overexpressing plants was around two-fold higher than wild type. Moreover, transgenic plants had higher numbers of branches (52.4%) and greater branch lengths (60–203%), but smaller leaf size (77.6%). Interestingly, relative to wild type the number and size of glandular trichomes in transgenic leaves was about 30 and 35% higher, respectively. From GC–MS analysis, the proportion of diterpenes in transgenic plant extracts was 1.5-fold lower than those noticed in wild type, while the proportion of sesquiterpenes was increased about 1.6 times when compared to wild type. However, the content proportion of monoterpenes showed a slightly increase, whereas the level of triterpenes showed no variation. In addition, two monoterpenes (eucalyptol and borneol), four sesquiterpenes (α-caryophyllene, β-guaiene, δ-cadinene and β-cubebene) and one triterpenes (isomultiflorenone) were detected only in transgenic extract, whereas d-α-tocopherol, a diterpenoid compound was found only in wild type but not transgenic plant. These results suggested that gibberellins play a significant role in regards to morphology, trichome formation and terpenoid metabolite production in A. annua. © 2017, Springer Science+Business Media Dordrecht.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010818020&doi=10.1007%2fs11240-017-1171-1&partnerID=40&md5=ce8946ae2b28e61275c47e2e6e445939
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Plants
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Overexpression of the gibberellin 20-oxidase gene from Torenia fournieri resulted in modified trichome formation and terpenoid metabolities of Artemisia annua
Inthima P.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Erratum to: Overexpression of the gibberellin 20-oxidase gene from Torenia fournieri resulted in modified trichome formation and terpenoid metabolities of Artemisia annua L. (Plant Cell, Tissue and Or Horticultural characterization of a tetraploid transgenic plant of Tricyrtis sp. Carrying the gibberellin 2-oxidase gene T-DNA insertion alters the terpenoid content composition and bioactivity of transgenic Artemisia annua Mutagenic effects of heavy-ion beam irradiation on in vitro nodal segments of Artemisia annua L. การกระจายตัวของลักษณะทางสัณฐานวิทยาของแววมยุราลูกผสม (Torenia fournieri x Torenia baillonii) ที่เป็นเตตระพลอยด์ในประชากรรุ่นที่ 2 ต้นทุนการผลิตยางแท่งชั้น 20 (STR 20) Gene co-expression analysis inferring the crosstalk of ethylene and gibberellin in modulating the transcriptional acclimation of cassava root growth in different seasons Cloning of Arabidopsis WUS Gene, Construcion and Identification of WUS-EGFP Fusion Gene Plant Expression Vector Effect of Insulin like growth factor 1 gene, Insulin like growth factor 2 gene and Melanocortin 4 receptor gene on growth performance and carcass traits in Thai indigenous chicken (Leung Hang Khao) Genome-wide prediction of the polymorphic Ser gene family in Tetrahymena thermophila based on motif analysis
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