สืบค้นงานวิจัย
Development and validation of LC-MS/MS method for quantitative determination of adenosine, guanosine, xanthine and uric acid in widely consumed vegetables in Thailand
Rukdee N. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Development and validation of LC-MS/MS method for quantitative determination of adenosine, guanosine, xanthine and uric acid in widely consumed vegetables in Thailand
ผู้แต่ง / หัวหน้าโครงการ (EN): Rukdee N.
บทคัดย่อ (EN): In this study, a triple quadrupole LC-MS/MS electrospray ionization method was developed and validated for quantitative determination of adenosine, guanosine, xanthine and uric acid in fifteen widely consumed Thai vegetables. The method was successively developed by using caffeine as internal standard. The lower limit of quantitation (LLOQ) was 0.2 μg/g for adenosine and guanosine, and 1.0 μg/g for uric acid and xanthine. The method was fully validated according to USFDA guidelines and all performance characteristics were found acceptable. Subsequently, the developed and validated LC-MS/MS method was applied to determine the four interest substances in fifteen widely consumed vegetables in Thailand. The results showed that all vegetables included in the study could be classified as low adenosine, guanosine, xanthine and uric acid containing foods since the concentrations of these substances were less than 50 mg per 100 g. This finding was enormously valuable information for hyperuricemia and gouty patients.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84945999047&partnerID=40&md5=c4c7680d7ce081c613c911c4385ccbc9
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Xanthine
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Development and validation of LC-MS/MS method for quantitative determination of adenosine, guanosine, xanthine and uric acid in widely consumed vegetables in Thailand
Rukdee N.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
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