สืบค้นงานวิจัย
Association of Melatonin receptor 1A (MTNR1A) gene polymorphism with seasonal suppression of fertility in buffaloes (Buffalo Bulletin,41(4))
Ranjna Cheema - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Association of Melatonin receptor 1A (MTNR1A) gene polymorphism with seasonal suppression of fertility in buffaloes (Buffalo Bulletin,41(4))
ผู้แต่ง / หัวหน้าโครงการ (EN): Ranjna Cheema
หน่วยงานสังกัดผู้แต่ง (EN): Ludhiana (India). Department of Veterinary Gynaecology and Obstetrics
บทคัดย่อ (EN): The polymorphism in Melatonin receptor 1A (MTNR1A) gene and its association with reproductive cyclicity (cyclic, n=51, non-cyclic, n=50) during summer season and age at first calving (LT 3.5 years, n=38, GT 3.5 years, n=60) was investigated in buffaloes subjected to blood sampling. Polymerase chain reaction (PCR) of genomic DNA was carried out using three primers viz. 824 bp (primer pair 1), 856 bp (primer pair 2) and 268 bp (primer pair 3) corresponding to exon-II of MTNR1A gene. Restriction fragment length polymorphism was performed using PCR products of primer pair 1 and 2. The digestion of PCR product of primer pair 1 with restriction enzymes viz. MnII, RsaI or Eco3II generated total restriction sites respectively as 14, 4 and 5. Based upon differential restriction sites, six genotypes revealed in buffaloes using MnII were MM-1 to MM-6, four genotypes using RsaI were RR-1 to RR-4, and four genotypes using Eco3II were EE-1 to EE-4. The digestion of PCR product of primer pair 2 with MnII, RsaI or Eco3II generated restriction sites respectively as 13, 5 and 6. Based upon differential restriction sites, six genotypes revealed using MnII were mm-1 to mm-6, five genotypes using RsaI were rr-1to rr-5, and three genotypes using Eco3II were ee-1 to ee-3. For primer pair 3, single stranded conformational polymorphism analysis exhibited 5 genotypes viz. AA, BB, AB, CC and DD. The results revealed that the buffaloes during summer had polymorphic genotypes for MTNR1A gene as buffaloes showing reproductive cyclicity had MM-3, MM-4, RR-2, EE-3, mm-2, mm-4, rr-3, ee-3, CC and DD genotypes, whereas their non-cyclic counterparts had MM-2, MM-6, EE-4, mm-3, mm-6, AA, BB and AB genotypes. However, no such differences were recorded with respect to age at first calving. In conclusion, polymorphic genotypes of MTNR1A gene may be considered as genetic markers to identify buffaloes with better reproductive potential during summer season.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/422539
คำสำคัญ (EN): Summer
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Ranjna Cheema. "Association of Melatonin receptor 1A (MTNR1A) gene polymorphism with seasonal suppression of fertility in buffaloes (Buffalo Bulletin,41(4))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Ranjna Cheema. "Association of Melatonin receptor 1A (MTNR1A) gene polymorphism with seasonal suppression of fertility in buffaloes (Buffalo Bulletin,41(4))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Association of Melatonin receptor 1A (MTNR1A) gene polymorphism with seasonal suppression of fertility in buffaloes (Buffalo Bulletin,41(4))
Ranjna Cheema
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Melanocortin 1 receptor (MC1R) gene polymorphism and its association with coat colour patterns in Nagpuri and Purnathadi buffaloes (Buffalo Bulletin,41(3)) Expression and structural variations of toll-like receptor 4 gene in buffalo and cattle (Buffalo Bulletin,41(1)) Molecular characterization of buffalo alpha s1-casein gene (Buffalo Bulletin,41(3)) Incidence of various haemoprotozoan diseases in buffaloes (Buffalo Bulletin,41(3)) Clinico-biochemical and therapeutic management of mammitis in buffaloes (Buffalo Bulletin,41(2)) Efficacy of antioxidant in the therapy of subclinical mastitis in buffaloes (Buffalo Bulletin,41(1)) Ultrasonography and biochemical studies of hepatobiliary system in buffaloes (Buffalo Bulletin,41(2)) Aquatic fern Azolla affecting milk production in buffaloes (Buffalo Bulletin,41(4)) Diagnosis and therapeutic management of oral tumours in Murrah buffaloes (Buffalo Bulletin,41(1)) Estimates of genetic parameters for production and reproduction traits in Murrah buffaloes (Riverine buffalo) in India (Buffalo Bulletin,41(1))
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