สืบค้นงานวิจัย
Studies on Agrobacterium-mediated Genetic Transformation of Embryogenic Cell Suspension and Plant Regeneration in Hevea brasiliensis
Zehai Jiang - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Studies on Agrobacterium-mediated Genetic Transformation of Embryogenic Cell Suspension and Plant Regeneration in Hevea brasiliensis
ผู้แต่ง / หัวหน้าโครงการ (EN): Zehai Jiang
บทคัดย่อ (EN): The establishment of embryogenic cell suspension culture and plant regeneration system of Hevea brasiliensis can provide sufficient and fine materials for studying genetic transformation. In this study, the plant expression vector pCAMBIA2301 containing uidAgene, npt-II gene and AtCBF1 gene in the T-DNA region was transformed into Agrobactrium tumefaciens EHA105. The embryogenic suspension cells of Chinese rubber tree clone Reyan 8-79 were used as transformation recipients. The Agrobacterium-mediated genetic transformation had been carried out. The factors influencing genetic transformation were studied. The frequency of transient expression of uidA gene and the survival rate of suspension cells were used as indexes. The optimal conditions for Agrobacterium-mediated transformation of embryogenic cell suspensions of rubber tree were determined as the following: the bacterial suspension OD600 was about 0.3 to 0.5, the pH value of co-culture medium was around 5.2, 100-150 iM acetosyringone supplemented in co-culture media, the co-culture temperature was about 20 to 22 !, about 4-5 d co-cultured on solid co-culture medium, Ticarcillin with concentration of 300 mg/L was used to inhibit Agrobacterium after co-culture, and kanamycin concentration gradient from low to high (50, 75, 100 mg/L) was used to select resistant embryogenic callus of rubber tree. Total of 10 lines of resistant embryogenic callus, 2177 putative transgenic embryoids and 165 putative transgenic plantlets were obtained. GUS staining, PCR identification, putative transgenic plantlets PCR products sequencing analysis and Southern blotting analysis were used to identify the resistant embryogenic calli, embryoids and regenerated plants. The results showed that transgenic plantlets had been obtained. The exogenous genes such as npt-II had been successfully integrated into the genomic DNA of the transgenic plantlets of rubber tree. In this study, the Agrobacterium-mediated genetic transformation system with embryogenic suspension cells of rubber tree as recipients had been preliminarily established. It will be beneficial to gene engineering breeding and gene function studies of rubber tree.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เผยแพร่โดย (EN): การยางแห่งประเทศไทย
คำสำคัญ (EN): embryogenic callus
เจ้าของลิขสิทธิ์ (EN): การยางแห่งประเทศไทย
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Zehai Jiang. "Studies on Agrobacterium-mediated Genetic Transformation of Embryogenic Cell Suspension and Plant Regeneration in Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.

Zehai Jiang. "Studies on Agrobacterium-mediated Genetic Transformation of Embryogenic Cell Suspension and Plant Regeneration in Hevea brasiliensis". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Studies on Agrobacterium-mediated Genetic Transformation of Embryogenic Cell Suspension and Plant Regeneration in Hevea brasiliensis
Zehai Jiang
การยางแห่งประเทศไทย
ไม่ระบุวันที่เผยแพร่
Predicting the distribution of rubber trees (Hevea brasiliensis) through ecological niche modelling with climate, soil, topography and socioeconomic factors Functional analysis of HbMlo12 in powdery mildew infection and stress response in the rubber tree Hevea brasiliensis Muell Arg. Agrobacterium Strain- A Key Factor Determining the Efficiency of Transformation in Hevea brasiliensis Plant Hormones and Oxidative Stress in Hevea brasiliensis Genetic Transformation and Regeneration of Hevea Brasiliensis Transgenic Plant with GAI Gene Studies on Cold Tolerance of Hevea brasiliensis (Muell. Arg.) Clones under Controlled Environmental Conditions Cloning and expression of HbACO2 of Hevea brasiliensis Localization of Peroxidase Enzyme in the Bark of Hevea brasiliensis MicroRNAs: New Regulators of Biological Functions in Hevea brasiliensis Some Considerations Concerning the Yield Potential of Some Clones (Hevea brasiliensis)
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