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Morphological, histopathological and secondary structure analysis of second internal transcribed spacer (ITS-2) region of Gigantocotyle explanatum (Trematoda: Paramphistomidae) in buffaloes of Pakistan (Buffalo Bulletin,41(1))
Ghulam Narjis - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Morphological, histopathological and secondary structure analysis of second internal transcribed spacer (ITS-2) region of Gigantocotyle explanatum (Trematoda: Paramphistomidae) in buffaloes of Pakistan (Buffalo Bulletin,41(1))
ผู้แต่ง / หัวหน้าโครงการ (EN): Ghulam Narjis
หน่วยงานสังกัดผู้แต่ง (EN): Islamabad (Pakistan). Faculty of Natural Sciences. Department of Statistics
บทคัดย่อ (EN): Liver infections due to amphistomes in buffaloes cause significant economic losses in livestock sector. The present study investigated the classical morphological features of adult amphistomes combined with histopathology and molecular identity in slaughtered buffaloes from Khyber Pakhtunkhwa, Pakistan. Adult amphistome were collected and morphologically characterized as Gigantocotyle explanatum. Morphometric measurement (n=50) were obtained with 12.05+-1.68 mm in length and 5.77+-0.81 mm in width, and the values of sagittal section was 6.35+-1.03 * 2.98+-0.50 mm in size. Severe bile duct pathology was observed with fibrosis of the duct wall with an irregular epithelial border, hyperplasia and inflammatory response with numerous neutrophils and eosinophils. The molecular identity of G. explanatum within Paramphistomidae was confirmed by ITS-2 rDNA sequences phylogenetic analysis based on maximum likelihood method. The genetic data based on ITS-2 secondary structure of G. explanatum consisted of four helix, Helix I, II and IV were conserved as compared with other closely related reference taxa of family Paramphistomidae and Gastrothylacidae. Helix III expressed some variations. The study concluded that rDNA ITS-2 and secondary structure information provides a guide for other researchers to determine the molecular taxonomic position of Paramphistomidae trematodes, data will support future clinical studies and control measures to reduce the amphistomiasis in buffaloes.
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ภาษา (EN): en
เอกสารแนบ (EN): https://kukr.lib.ku.ac.th/db/kukr/search_detail/result/416655
คำสำคัญ (EN): Pakistan
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Ghulam Narjis. "Morphological, histopathological and secondary structure analysis of second internal transcribed spacer (ITS-2) region of Gigantocotyle explanatum (Trematoda: Paramphistomidae) in buffaloes of Pakistan (Buffalo Bulletin,41(1))". ไม่ระบุหน่วยงาน. ม.ป.ป.

Ghulam Narjis. "Morphological, histopathological and secondary structure analysis of second internal transcribed spacer (ITS-2) region of Gigantocotyle explanatum (Trematoda: Paramphistomidae) in buffaloes of Pakistan (Buffalo Bulletin,41(1))". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Morphological, histopathological and secondary structure analysis of second internal transcribed spacer (ITS-2) region of Gigantocotyle explanatum (Trematoda: Paramphistomidae) in buffaloes of Pakistan (Buffalo Bulletin,41(1))
Ghulam Narjis
ไม่ระบุผู้เผยแพร่
ไม่ระบุวันที่เผยแพร่
The Population structure analysis in Sumbawa buffaloes (Bubalus bubalis) of Indonesia (Buffalo Bulletin,41(3)) Incidence of various haemoprotozoan diseases in buffaloes (Buffalo Bulletin,41(3)) Efficacy of antioxidant in the therapy of subclinical mastitis in buffaloes (Buffalo Bulletin,41(1)) Cystadenocarcinoma of Bartholin's gland - Histopathological and immunohistochemical study (Buffalo Bulletin,41(3)) Ultrasonography and biochemical studies of hepatobiliary system in buffaloes (Buffalo Bulletin,41(2)) Clinico-biochemical and therapeutic management of mammitis in buffaloes (Buffalo Bulletin,41(2)) Aquatic fern Azolla affecting milk production in buffaloes (Buffalo Bulletin,41(4)) Diagnosis and therapeutic management of oral tumours in Murrah buffaloes (Buffalo Bulletin,41(1)) Estimates of genetic parameters for production and reproduction traits in Murrah buffaloes (Riverine buffalo) in India (Buffalo Bulletin,41(1)) Analysis of export potential of buffalo meat in India (Buffalo Bulletin,40(4))
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