สืบค้นงานวิจัย
Characterization of the commercially-available fluorescent chloroquine-BODIPY conjugate, LynxTag- CQGREEN, as a marker for chloroquine resistance and uptake in a 96-well plate assay
Loh C.C.Y. - ไม่ระบุหน่วยงาน
ชื่อเรื่อง (EN): Characterization of the commercially-available fluorescent chloroquine-BODIPY conjugate, LynxTag- CQGREEN, as a marker for chloroquine resistance and uptake in a 96-well plate assay
ผู้แต่ง / หัวหน้าโครงการ (EN): Loh C.C.Y.
บทคัดย่อ (EN): Chloroquine was a cheap, extremely effective drug against Plasmodium falciparum until resistance arose. One approach to reversing resistance is the inhibition of chloroquine efflux from its site of action, the parasite digestive vacuole. Chloroquine accumulation studies have traditionally relied on radiolabelled chloroquine, which poses several challenges. There is a need for development of a safe and biologically relevant substitute. We report here a commercially-available green fluorescent chloroquine-BODIPY conjugate, LynxTag-CQ GREEN, as a proxy for chloroquine accumulation. This compound localized to the digestive vacuole of the parasite as observed under confocal microscopy, and inhibited growth of chloroquine-sensitive strain 3D7 more extensively than in the resistant strains 7G8 and K1. Microplate reader measurements indicated suppression of LynxTag-CQGREEN efflux after pretreatment of parasites with known reversal agents. Microsomes carrying either sensitiveor resistant-type PfCRT were assayed for uptake; resistant-type PfCRT exhibited increased accumulation of LynxTag-CQGREEN, which was suppressed by pretreatment with known chemosensitizers. Eight laboratory strains and twelve clinical isolates were sequenced for PfCRT and Pgh1 haplotypes previously reported to contribute to drug resistance, and pfmdr1 copy number and chloroquine IC50s were determined. These data were compared with LynxTag-CQGREEN uptake/fluorescence by multiple linear regression to identify genetic correlates of uptake. Uptake of the compound correlated with the logIC50 of chloroquine, more weakly, a mutation in Pgh1, F1226Y. ©2014 Loh et al.
บทคัดย่อ: ไม่พบข้อมูลจากหน่วยงานต้นทาง
ภาษา (EN): en
เอกสารแนบ (EN): https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908425701&doi=10.1371%2fjournal.pone.0110800&partnerID=40&md5=4bde09ac32db4abf50a657c82bf6d71c
เผยแพร่โดย (EN): มหาวิทยาลัยมหิดล
คำสำคัญ (EN): Verapamil
เจ้าของลิขสิทธิ์ (EN): มหาวิทยาลัยมหิดล
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Loh C.C.Y.. "Characterization of the commercially-available fluorescent chloroquine-BODIPY conjugate, LynxTag- CQGREEN, as a marker for chloroquine resistance and uptake in a 96-well plate assay". ไม่ระบุหน่วยงาน. ม.ป.ป.

Loh C.C.Y.. "Characterization of the commercially-available fluorescent chloroquine-BODIPY conjugate, LynxTag- CQGREEN, as a marker for chloroquine resistance and uptake in a 96-well plate assay". ไม่ระบุหน่วยงาน. ม.ป.ป.. Print.



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Characterization of the commercially-available fluorescent chloroquine-BODIPY conjugate, LynxTag- CQGREEN, as a marker for chloroquine resistance and uptake in a 96-well plate assay
Loh C.C.Y.
มหาวิทยาลัยมหิดล
ไม่ระบุวันที่เผยแพร่
Chloroquine is grossly under dosed in young children with malaria: Implications for drug resistance Prevalence of primary HIV drug resistance in Thailand detected by short reverse transcriptase genotypic resistance assay Characterization of antibiotic resistance in Escherichia coli isolated from shrimps and their environment PHOSPHORUS UPTAKE BY RUBBER (HEVEA BRASILIENSIS) MODIFICATIONS AT THE RHIZOSPHERE FOR IMPROVED UPTAKE Use of dried blood spot specimens for in-house HIV drug resistance genotyping assay among infants in Thailand Genetic diversity of the Plasmodium vivax multidrug resistance 1 gene in Thai parasite populations Functional characterization of CgPbs2, a MAP kinase kinase , in Colletotrichum gloeosporioides by using the osmotic stress sensibility as selection marker Molecular assays for antimalarial drug resistance surveillance: A target product profile Prevalence of Plasmodium falciparum molecular markers of antimalarial drug resistance in a residual malaria focus area in Sabah, Malaysia Available phosphorus declines in organic residues-treated sandy soils indicating its transformation to more slowly available forms for phosphorus conservation
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